地锦草片对免疫性血小板减少症患者T淋巴细胞亚群及细胞因子的影响  

Effects of Dijincao Tablets on T-lymphocytes subsets and cytokines in patients with immune thrombocytopenia

作　　者：卢秀花[1] 陈忠光[1] 王彦丽[2] 

机构地区：[1]临沂市沂水中心医院药学部,山东临沂276400 [2]临沂市沂水中心医院血液内科,山东临沂276400

出　　处：《中成药》2017年第1期50-54,共5页Chinese Traditional Patent Medicine

摘　　要：目的观察地锦草片(地锦草)对免疫性血小板减少症患者淋巴细胞亚群及细胞因子的影响。方法选取2014.1—2015.12我院血液科诊断免疫性血小板减少症首次住院治疗的56例患者,采用分层区组随机化方法分为对照组(28例)和观察组(28例),对照组按照指南给予常规治疗,观察组在常规治疗的基础上给予地锦草片(4.8 g,tid),治疗过程中收集不良事件,所有患者分别于入院第2天及第4周末(入院第30天)抽取清晨空腹静脉血检测观察指标并评价综合疗效。运用流式细胞仪检测淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+水平,用ELISA法检测血清细胞因子IL-2、IFN-γ、IL-4、IL-10水平。结果观察组总有效率为92.9%,高于对照组(82.1%),观察组血小板水平明显高于对照组。与对照组相比,观察组CD3+、CD4+、CD4+/CD8+水平明显升高,IL-2和IFN-γ水平明显降低,IFN-γ/IL-4比值明显降低。结论地锦草片能纠正T淋巴细胞亚群的紊乱,调节T淋巴细胞亚群比例,降低IFN-γ/IL-4的比值,达到治疗免疫性血小板减少症患者作用。AIM To observe the effects of Dijincao Tablets（ Euphorbia humifusa Willd.） on T-lymphocytes subsets and cytokines in patients with immune thrombocytopenia（ ITP）. METHODS Fifty-six patients who were first diagnosed ITP at hematology department of our hospital from Jan. 2014 to Dec. 2015 were divided into observation group（ twenty-eight cases） and control group（ twenty-eight cases） by Stratified blocked randomization. The patients in the observation group were given Dijincao Tablets（ 4. 8 g,tid） based on the conventional treatment,while the patients in the control group only accepted conventional treatment according to guidelines. Adverse events were collected during the treatment. Fasting venous blood was obtained from all patients in the morning on the second day of admission and the weekend of the forth week（ 30 thday after admission） to detect observation indexes,and then the comprehensive effects were evaluated. The levels of T-lymphocytes subsets of CD3＋,CD4＋,CD8＋and CD4＋/ CD8＋were examined by flow cytometry,and serum cytokine levels of IL-2,IFN-γ,IL-4 and IL-10 were determined by ELISA. RESULTS The total effective rate was 92. 9% in the observation group,which was higher than 82. 1% in the control group. The platelet level in the observation group was significantly higher than that in the control group. Compared with the control group,the levels of CD3＋,CD4＋and CD4＋/ CD8＋in the observation group were significantly increased,the levels of IL-2 and IFN-γ were significantly decreased,and the ratio of IFN-γ / IL-4 was significantly decreased. CONCLUSION Dijincao Tablets can correct and regulate the disorder and ratio of T-lymphocyte subsets,and decrease the ratio of IFN-γ / IL-4,then achieve therapeutic effect on patients with immune thrombocytopenia.

关 键 词：地锦草片 免疫性血小板减少症 T淋巴细胞亚群 细胞因子 

分 类 号：R287[医药卫生—中药学]