GC-FID法同时测定开喉剑喷雾剂中4种成分  

Simultaneous determination of four constituents in Kaihoujian Spray by GC-FID

作　　者：杜贵琴[1] 左雅敏[1] 潘卫[1] 李兴[1] 田红红[2] 伍庆[2] 杜莹[2] 

机构地区：[1]贵州医科大学医学检验学院,贵州贵阳550004 [2]贵州师范大学,贵州贵阳550001

出　　处：《中成药》2017年第1期94-97,共4页Chinese Traditional Patent Medicine

基　　金：喀斯特地区生物与信息技术协同创新中心(黔教合协同创新字[2014]04);贵州省科技成果转化引导基金计划项目(黔科合成转字[2015]2015-2);贵州省科学技术基金(黔科合J字[2014]2002)

摘　　要：目的建立气相色谱-氢火焰离子化检测器(GC-FID)法同时测定开喉剑喷雾剂(山豆根、蝉蜕、薄荷脑等)中4种成分的含有量。方法开喉剑喷雾剂和内标(邻苯二甲酸二丁酯)溶液混合物的分析采用HP-INNOWax色谱柱(30 m×0.25 mm×0.25μm);进样口温度260℃;检测器温度260℃;分流比5∶1;体积流量1.5 m L/min。结果薄荷脑、苦参碱、槐果碱、槐定碱分别在0.228 7~2.287 0 mg/m L(r=0.999 7)、0.011 1~0.111 0 mg/m L(r=0.999 6)、0.016 8~0.168 0 mg/m L(r=0.999 8)、0.003 4~0.034 0 mg/m L(r=0.999 4)范围内呈良好的线性关系,平均加样回收率(n=9)分别为98.4%(RSD=1.2%)、95.9%(RSD=1.8%)、96.2%(RSD=2.1%)、97.1%(RSD=1.7%)。结论该方法简单准确,灵敏度高,可用于开喉剑喷雾剂的质量控制。AIM To establish a gas chromatography-flame ionization detector（ GC-FID） method for the simultaneous content determination of four constituents in Kaihoujian Spray（ a spray for management of oral and laryngopharyngeal inflammatory condition,containing Sophorae tonkinensis Radix et Rhizoma,Cicadae Periostracum,menthol,etc.）. METHODS The analysis of mixure of Kaihoujian Spray and internal standard（ dibutyl phthalate）solution was conducted on an HP-INNOWax column（ 30 m × 0. 25 mm × 0. 25 μm）,both of the injector temperature and detector temperature were set at 260 ℃,the split ratio was 5 ∶ 1,and the flowing rate was 1. 5 m L / min.RESULTS Menthol,matrine,sophocarpine and sophoridine showed good linear relationships within the ranges of0. 228 7-2. 287 0 mg / m L（ r = 0. 999 7）,0. 011 1-0. 111 0 mg / m L（ r = 0. 999 6）,0. 016 8-0. 168 0 mg / m L（ r = 0. 999 8） and 0. 003 4-0. 034 0 mg /m L（ r = 0. 999 4）,whose average recoveries（ n = 9） were 98. 4%（ RSD = 1. 2%）,95. 9%（ RSD = 1. 8%）,96. 2%（ RSD = 2. 1%） and 97. 1%（ RSD = 1. 7%）,respectively.CONCLUSION This accurate,sensitive and simple method can be used for the quality control of Kaihoujian Spray.

关 键 词：开喉剑喷雾剂 薄荷脑 苦参碱 槐果碱 槐定碱 GC-FID 

分 类 号：R927.2[医药卫生—药学]