HPLC法同时测定咳速停胶囊中4种成分  

Simultaneous determination of four constituents in Kesuting Capsules by HPLC

作　　者：罗奕[1,2] 姚文丽[1] 吴琳琳[1] 杨娟艳 茅向军[2] 

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]贵州省食品药品检验所,贵州贵阳550004 [3]贵阳中医学院,贵州贵阳550002

出　　处：《中成药》2017年第1期102-106,共5页Chinese Traditional Patent Medicine

基　　金：基金项目:国家药典委员会2015年度药品标准提高工作(87)

摘　　要：目的建立HPLC法同时测定咳速停胶囊(麻黄、罂粟壳、桔梗等)中4种成分的含有量。方法咳速停胶囊三氯甲烷-浓氨提取液的分析采用Welch Ultimate~?XB-C_(18)色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.01 mol/L磷酸二氢钾缓冲液为流动相,等度洗脱;体积流量1.0 m L/min;柱温35℃;检测波长210 nm。结果吗啡、盐酸麻黄碱、盐酸伪麻黄碱、磷酸可待因分别在8.054~67.12μg/m L(r=0.999 5)、22.31~185.9μg/m L(r=0.999 9)、21.26~177.2μg/m L(r=0.999 7)、1.212~10.09μg/m L(r=0.999 7)范围内线性关系良好,平均加样回收率(n=9)分别为100.9%、101.4%、105.3%、106.2%,RSD分别为2.0%、3.6%、1.2%、1.2%。结论该方法快速简便,可用于咳速停胶囊的质量控制。AIM To establish an HPLC method for the simultaneous content determination of four constituents in Kesuting Capsules（ a fast cough suppressant,containing Ephedrae Herba,Papaveris Pericarpium,Platycodonis Radix,etc.）. METHODS The analysis of trichloromethane-strong ammonia extract of Kesuting Capsules was performed on a 35 ℃ thermostatic Welch Ultimate-？XB-C（18） column（ 4. 6 mm × 250 mm,5 μm）,with the mobile phase comprising of acetonitrile-0. 01 mol / L potassium dihydrogen phosphate buffer flowing at 1. 0 m L / min in an isocratic elution manner,and the detection wavelength was set at 210 nm. RESULTS Morphine,ephedrine hydrochloride,pseudoephedrine hydrochloride and codeine phosphate showed good linear relationships within the ranges of 8. 054-67. 12 μg / m L（ r = 0. 999 5）,22. 31-185. 9 μg / m L（ r = 0. 999 9）,21. 26-177. 2 μg / m L（ r = 0. 999 7） and 1. 212-10. 09 μg /m L（ r = 0. 999 7）,whose average recoveries（ n =9） were 100. 9%（ RSD =2. 0%）,101. 4%（ RSD = 3. 6%）,105. 3%（ RSD = 1. 2%） and 106. 2%（ RSD = 1. 2%）,respectively. CONCLUSION This simple method can be used for the rapid quality control of Kesuting Capsules.

关 键 词：咳速停胶囊 吗啡 盐酸麻黄碱 盐酸伪麻黄碱 磷酸可待因 HPLC 

分 类 号：R927.2[医药卫生—药学]