国槐氮素转运对干旱胁迫的分子响应机制  

Mechanism of Molecular Responses of Nitrogen Transport to Drought Stress in Sophora japonica

作　　者：苏莉[1] 孟森[1] 张胜[1] 李怡鸣 赵忠[1,2] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]教育部西部环境与生态重点实验室,陕西杨陵712100

出　　处：《西北林学院学报》2017年第1期1-11,18,共12页Journal of Northwest Forestry University

基　　金：林业公益性行业专项"黄河中游地区Ⅰ级古树名木保护关键技术研究"项目(201404302)

摘　　要：为了从分子水平上了解国槐(Sophora japonica)氮素转运对干旱胁迫的响应机制,以国槐为对象,参考大豆(Glycine max)和鹰嘴豆(Cicer arietinum)氮素转运同源基因,设计国槐氮素转运蛋白基因克隆引物,克隆国槐氮转运蛋白基因;测定不同程度(轻度、中度、重度)干旱胁迫下国槐幼苗氮素转运蛋白基因相对表达量。结果表明,通过同源克隆得到5条氮素转运蛋白基因,SjNPF2.11、SjNPF2.13、SjNPF7.2、SjAMT1.3和SjAMT2.1,基因片段长度分别为424、490、294、407、577bp。其中SjNPF2.13在国槐根部的表达量在中度和重度胁迫36h后开始显著上升;叶部的表达量在中度和重度胁迫24h后开始显著上升,轻度胁迫48h后表达量显著上升;SjNPF7.2根部的表达量在中度和重度胁迫24h后开始显著上升;叶部的相对表达量在重度干旱胁迫24h和48h时显著下降;SjAMT1.3根部的表达量在各干旱胁迫处理24h和36h后显著上升。叶部的表达量在重度干旱胁迫12h后开始显著下降。试验表明,SjAMT1.3可能参与干旱胁迫下国槐根系对铵盐的吸收,而SjNPF2.13和SjNPF7.2可能参与干旱胁迫下硝酸盐在国槐体内的转运和同化。In order to understand the nitrogen transport respond mechanism of Sophora japonica to drought stress at the molecular level,S, japonica was used as material to clone nitrogen transporters and to analyze the expression characteristics under drought stress. Clone primers were designed referring to the homologous genes of Glycine max and Cicer arietinum, then S. japonica was used as material to clone nitrogen transporters and to determine the relative expression of S. japonica seedlings under varying drought degrees （mild,moderate and severe）. Five nitrogen transporters were cloned from S. japonica by homologous clone,SjNPF2.11, SjNPF2.13, SjNPF7.2, SjAMT1.3 and SjAMT2. 1 with genic sequence lengths of 424 hp,490 bp,294 bp,407 bp,577 bp,respectively. SjNPF2.13 expression in S. japonica roots began to rise significantly after moderate and severe drought treatment for 36 h,but in leaves it began to rise significantly after treatment for 24 h,and it also began to rise significantly after mild drought treatment for 48 h. SjNPF7.2 expression in roots began to rise significantly after moderate and severe drought treatment for 24 h,but in leaves it decreased significantly after severe drought treatment for 12 h and 48 h. SjAMT1.3 expression in roots began to rise significantly after drought treatment for 24 h,but in leaves it began to decrease significantly after drought treatment for 12 h. The results indicated that SjAMT1.3 participated in the ammonium uptake in S. japonica roots under drought dress,while SjNPF2.13 and SjNPF7.2 participated in the nitrate transloeation and assimilation in vivo under drought stress.

关 键 词：国槐 氮素转运蛋白 同源克隆 干旱胁迫 

分 类 号：S562.01[农业科学—作物学]