银杏叶提取物缓释剂穴位埋药线对局灶性脑缺血再灌注大鼠脑组织损伤及Nrf2/HO-1信号通路的影响  

Ginkgo biloba Extract Release Agent Acupoint Embedding Therapy Attenuates the Injury of Cerebral Ischemia/Reperfusion through Nrf2/HO-1 Signal Pathway

作　　者：杨静 吴凤芝[2] 李瑜霞 张冬森 

机构地区：[1]唐山市工人医院中医科,唐山063003 [2]北京中医药大学,北京100029 [3]唐山市工人医院神经内科,唐山063003

出　　处：《天然产物研究与开发》2017年第1期27-33,共7页Natural Product Research and Development

基　　金：河北省中医药管理局计划(2016130)

摘　　要：银杏叶提取物缓释剂穴位埋药线对局灶性脑缺血再灌注损伤大鼠脑组织损伤及其对Nrf2/HO-1信号通路的影响。采用改良线栓法制备脑缺血再灌注大鼠模型,银杏叶提取物缓释剂穴位埋药线后进行神经缺失症状评分,测定神经细胞病理学,检测丙二醛(MDA)、一氧化氮(NO)、超氧化物歧化酶(SOD)和谷胱甘肽(GSH)及脑组织Nrf2和HO-1 mRNA和蛋白的表达。结果表明银杏叶提取物缓释剂穴位埋药线可显著改善脑缺血再灌注模型动物的神经功能,提高神经细胞存活率,显著降低MDA、NO含量,升高总SOD活性和GSH含量,调节Nrf2/HO-1 mRNA和蛋白的表达水平。银杏叶提取物缓释剂穴位埋药线具有抗脑缺血再灌注损伤作用,其机制可能与与激活Nrf2/HO-1途径,促进了Nrf2的核转位,使HO-1等抗氧化物质表达上调,提高了机体对氧化损伤的抗性有关。The aim of this study was to observe the effects of Ginkgo biloba extract release agent acupoint embedding （GBER） therapy on brain injury and Nrf2/HO-1 signal pathway after cerebral ischemia/reperfusion in rats.Cerebral ischemia/reperfusion rat model was induced by the modified middle cerebral artery occlusion model （MCAO） method.The neurological deficit score and histopathological examination of hippocampal CA1 were performed.The levels of malondialdehyde （MDA）,nitric oxide （NO）,superoxide superoxide dismutase （SOD） and glutathione （GSH） were assayed.Nrf2/HO-1 mRNA and protein expressions of brain tissue were detected.The results showed that G.biloba extract sustained-release agent significantly increased the neurological deficit score,the number of neurons.GBER not only reduced the content of MDA and NO,but also augmented the activity of SOD and the content of GSH.The mRNA and protein of Nrf2/HO-1 in GBER group enhanced.These findings showed that GBER therapy attenuated the injury of cerebral ischemia/reperfusion via regulating Nrf2/HO-1 signal pathway.

关 键 词：脑缺血 再灌注损伤 银杏叶提取物 穴位埋线 NRF2 HO-1 

分 类 号：R284.2[医药卫生—中药学]