青海产三种特色果实多糖及其活性比较  

Crude Polysaccharides Extracted from Three Characteristic Plants in Qinghai Province and Their Activities

作　　者：张庆琳[1] 高慧琴[1] 凌丽君[1] 季崇敏 卢燕[1] 陈道峰[1] 

机构地区：[1]复旦大学药学院生药学教研室,上海201203 [2]青海博智生物科技有限公司,西宁810000

出　　处：《天然产物研究与开发》2017年第1期34-39,共6页Natural Product Research and Development

基　　金：科技部十二五"重大新药创制"重大科技专项(2012ZX09301001-003)

摘　　要：用水提醇沉法提取了青海产宁夏枸杞、黑枸杞、西伯利亚白刺三种特色果实的水溶性总多糖,利用理化方法测定并比较了三种多糖的理化性质及其抗氧化活性和对腹腔巨噬细胞NO分泌功能的影响。三种植物多糖分子量范围为130000~2350000;单糖组成都含有甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖及阿拉伯糖,但半乳糖醛酸、葡萄糖和阿拉伯糖的相对含量差异明显;三者的糖醛酸含量及蛋白质含量也有明显差异。活性测定结果表明,西伯利亚白刺多糖具有明显的DPPH清除能力,其IC50值达到0.19 mg/m L,表现出较强的抗氧化活性,而黑枸杞与宁夏枸杞多糖的活性较弱;三种多糖均可显著增强小鼠腹腔巨噬细胞的NO分泌水平,显示一定的免疫增强活性。The crude polysaccharides were extracted from the fruits of Lyciumbar barum L., L.ruthenicum Murr.and Nitrariasibirica Pall.,collected from Qinghai,by water extraction and ethanol precipitation.The three polysaccharides had relative molecular weight distribution of 130000 to 2350000,and all were composed of mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose,xylose and arabinose.However,the polysaccharides were quite different on their contents of galacturonic acid,glucose and arabinose,as well as the contents of uronic acid and protein.DPPH assay indicated that the polysaccharides of N.sibirica had more potent DPPH radical scavenging activity than those of L.ruthenicum and L.barbarum,with the IC50 value of 0.19 mg/mL.Besides,all the three crude polysaccharides dramatically promoted the NO secretion of macrophage,indicating that they had immuno-enhancing activity.

关 键 词：宁夏枸杞 黑枸杞 西伯利亚白刺 多糖 抗氧化 免疫增强 

分 类 号：R284.1[医药卫生—中药学]