不同类型柑橘中黄脉病毒衣壳蛋白基因的保守性及复制水平  

The Stability and Quantity of Coat Protein Gene of Citrus yellow vein clearing virus in Different Citrus Cultivars

作　　者：陈洪明[1] 马丹丹[1] 金鑫[1] 李太盛[1] 邓雨青 刘翠花[1] 吴强 李中安[1] 周彦[1] 

机构地区：[1]中国农业科学院柑橘研究所,西南大学柑橘研究所,国家柑橘工程技术研究中心,重庆400712 [2]安岳县柠檬科学技术研究所,四川安岳642300

出　　处：《园艺学报》2017年第1期106-112,共7页Acta Horticulturae Sinica

基　　金：重庆市基础与前沿研究计划重点项目(cstc2015jcyj BX0043);重庆科技支撑示范工程项目(cstc2014zktjccx BX0051);中央高校基本科研业务费项目(XDJK2015A009,2015C091,2014A001);重庆市两江学者项目

摘　　要：为研究不同柑橘品种对柑橘黄脉病毒(Citrus yellow vein clearing virus,CYVCV)变异的影响,将CYVCV毒株CQ01嫁接接种于4类共35个柑橘品种,对不同植株中CYVCV的衣壳蛋白(CP)基因进行分析,结果发现仅有少数位点发生了变异。运用RT-qPCR技术检测不同柑橘品种中CYVCV的相对含量,发现甜橙类品种中CYVCV的相对含量最高,墨西哥来檬中CYVCV的相对含量最低。植株中CYVCV的含量与其症状不存在明显的关联。For the preliminary study on the sequence variations of Citrus yellow vein clearing virus（CYVCV）by different citrus cultivars,CYVCV isolate CQ01 was grafted from Eureka lemon to 35 citrus cultivars. Sequence analysis on CP gene showed that few mutation was found among CYVCV isolates from different citrus cultivars. The results of RT-qPCR showed that the relative expression levels of CYVCV in sweet orange genotype were higher than in other citrus genotypes. The relative amount of CYVCV in Mexican lime was the lowest. Furthermore,there was no apparent relationship between the relative amount of CYVCV and the symptoms on citrus cultivar.

关 键 词：柑橘 柑橘黄脉病毒 品种 序列分析 RT-QPCR 

分 类 号：S666[农业科学—果树学]