糙皮侧耳凝集素基因Plectin的克隆和表达分析  

Cloning and Expression Analysis of Lectin Genes from Pleurotus ostreatus

作　　者：殷朝敏[1] 陈叶叶[2] 卢静[2] 马爱民[2] 

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所,武汉430064 [2]华中农业大学食品科技学院,武汉430070

出　　处：《园艺学报》2017年第1期131-138,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30771502;31172011)

摘　　要：基于cDNA末端快速扩增(RACE)技术克隆了两个糙皮侧耳凝集素基因Plectin1和Plectin2。结构分析显示Plectin1和Plectin2基因全长分别为1 371和1 359 bp,二者均含有5个外显子和4个内含子;Plectin1开放阅读框长1 134 bp,编码377个氨基酸,Plectin2开放阅读框长1 122 bp,编码373个氨基酸。Southern杂交试验证实Plectin1和Plectin2在糙皮侧耳基因组中均只有1个拷贝。利用qRT-PCR分析了Plectin1和Plectin2在糙皮侧耳不同生长阶段的表达量,结果显示Plectin1和Plectin2分别在成熟子实体阶段和幼嫩子实体阶段表达量最高,这表明Plectin1和Plectin2基因可能在糙皮侧耳子实体生长发育中起到一定的调控作用。Two lectin genes（Plectin1 and Plectin2） from Pleurotus ostreatus have been cloned based on rapid amplification of cDNA ends（RACE） method in this study. The full-lengths DNA of Plectin1 and Plectin2 are 1 371 bp,1 359 bp,and contain 5 exons and 4 introns,respectively. The open reading frame（ORF）of Plectin1 is 1 134 bp encoding 377 amino acids,while that of Plectin2 is 1 122 bp encoding 373 amino acids. Southern blot confirms both Plectin1 and Plectin2 had only one copy in the genome of P. ostreatus. qRT-PCR analysis shows Plectin1 and Plectin2 were highly expressed in mature fruiting body stage and young fruiting body stage respectively. This suggests Plectin1 and Plectin2 may play an important role in P. ostreatus fruiting body development.

关 键 词：糙皮侧耳 凝集素 SOUTHERN杂交 荧光定量PCR 

分 类 号：S646.141[农业科学—蔬菜学]