二倍体月季F1群体的SSR鉴定与遗传分析  

Hybrids Identification and Genetic Analysis in Diploid Roses Population(F_1)Using SSR Markers

作　　者：周宁宁[1] 李淑斌[1] 李远波[1] 蹇洪英[1] 晏慧君[1] 王其刚[1] 陈敏[1] 邱显钦[1] 张颢[1] 唐开学[1] 

机构地区：[1]云南省农业科学院花卉研究所,国家观赏园艺工程技术研究中心,云南省花卉育种重点实验室,昆明650205

出　　处：《园艺学报》2017年第1期151-160,共10页Acta Horticulturae Sinica

基　　金：云南省应用基础研究计划面上项目(2016FB061);云南省应用基础研究计划面上自筹项目(2013FZ148);国家自然科学基金项目(31160402)

摘　　要：以中国古老月季品种‘月月粉’(Rosa chinensis‘Old Blush’,用OB表示)为母本,‘无刺光叶蔷薇’(Rosa wichuriana‘Basye’s Thornless’,用W表示)为父本,构建F_1代共296个单株。从146对SSR标记中筛选出亲本间多态性好且条带清晰的23对SSR标记,对随机选择的94株F_1进行杂种鉴定和遗传分析。结果表明:筛选到3个纯合显性标记分别为Fv512、Fv609和305,可单独一次性鉴定全部杂种真实性;随机筛选的94株子代均为真杂种;20个SSR标记用于基因型分析,有9个标记出现了偏分离,并在统计上达到显著或极显著水平,偏分离率45%,说明该F_1群体基于SSR位点的基因型偏分离率较高,在进行高密度遗传图谱构建时应重视偏分离标记对作图的影响;UPGMA聚类分析显示,94株F_1的遗传变异大,且遗传多样性丰富,可划分为2个大类7个亚类。A segregating population of 296 F1 diploid hybrids has developed from a cross between Rosa chinensis‘Old Blush＇（OB）as maternal plant and Rosa wichuriana‘Basye＇s Thornless＇（W）as paternal plant. In this study,twenty-three SSR markers were found polymorphic within two parents among one hundred and forty-six SSR marks. We selected 94 progenies randomly from F1 population,used twenty-three SSR marks to test the authenticity of hybrids and the genetic relationship. Three homozygous dominant markers（Fv512,Fv609 and 305）were screened from the SSR marker sets,each marker is enough to identify the entire hybrids in our population. In total,all of progenies were identified as real interspecific hybrids,it suggested that the hybrid reliability is very high in our F1 population. Twenty markers were used to examine the genotype segregation,chi-square test indicated that nine（45%）markers exhibited segregation distortion with a significance levels at 5% and 1%,it suggested that F1 population have a high segregation distortion rate and the segregation distortion markers should be excluded when genetic linkage map construction. The UPGMA based dendrograms indicated that the genetic variation and diversity of 94 progenies was significant,and these progenies could be classified into 2 groups and 7 subgroups.

关 键 词：月季 二倍体 F1群体 杂种鉴定 SSR标记 偏分离 

分 类 号：S685.12[农业科学—观赏园艺]