屠宰肉鸡禽白血病病毒的检测及病理学观察  

Avian leukosis virus detection and liver pathology observation of slaughter broiler

作　　者：王靖媛[1] 程敏姮 佘锐萍[1] 吴桥兴[1] 石蕊寒[1] 胡凤姣[1] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]北京市动物疫病预防控制中心,北京102629

出　　处：《中国兽医科学》2017年第1期114-120,共7页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31472165)

摘　　要：为了了解肉鸡的健康状况以便对其进行食品安全的风险评估提供依据,本研究从北京某肉鸡屠宰场采集了300份肝、200份脾和100份腺胃,采用HE染色方法对各组织进行病理学观察,用免疫组织化学方法对肝组织中的禽白血病病毒J亚群(ALV-J)gp85特异性抗原进行检测,采用PCR技术对肝组织中的ALV RNA进行检测。结果显示,组织病理学观察可见肝中髓样细胞团块检出率为43%,淋巴细胞浸润病变占75%;脾见有少量淋巴细胞发生坏死、排空;52%腺胃黏膜坏死脱落,25%腺胃黏膜下有淋巴细胞浸润。免疫组织化学观察到肝样品中的ALV-J gp85抗原阳性率为61.7%,脾中阳性率为66.5%,腺胃样品阳性率为52.6%。PCR检测到ALV-J gp85特异性片段PCR产物阳性率为30.5%。利用Gen Bank比对发现与各株ALV-J的同源性为85%~90%。研究结果表明,该屠宰场肉鸡中存在着较高的ALV-J感染。To investigate the prevalence of avian leukosis subgroup J virus （ALV-J） among boiler chick- en in Beijng,we performed histopathology,immunohistochemistry and RT-PCR using 300 liver samples,200 spleen samples and 100 proventriculus samples. Histopathology test revealed a significant enhance on the number of neoplastic myeloid cells and lymphoid cells in the livers. Also,52%glandular stomach mucosashowed necrosis and exfoliation, ln immunohistochemistry analysis,the positive rates of ALV-J gp85 in the livers,spleens and proventriculus were 61.7%,66.5%and 52.6%,respectively. Through RT-PCR, we knew that gp85 gene positive rates in livers were 30.5%.The homologous rate between each ALV-J isolate was from 85%to 90%. Thus,our results provide evidence of ALV-J infection in boilr chicken in Beijng.

关 键 词：屠宰肉鸡 禽白血病病毒 组织病理学 免疫组织化学PCR 

分 类 号：S852.659.3[农业科学—基础兽医学]