鸡抗菌肽NK-lysin的生物信息学分析  

Bioinformatic analysis of antimicrobial peptide NK-lysin derived from chickens

作　　者：杭柏林[1] 李杰[2] 张慧辉[1] 徐彦召[1] 徐军[1] 胡建和[1] 

机构地区：[1]河南科技学院动物科技学院,河南新乡453003 [2]河南省新乡市畜产品质量监测检验中心,河南新乡453003

出　　处：《黑龙江畜牧兽医》2017年第1期29-31,36,291,292,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：河南省高校科技创新团队支持计划项目(15IRTS THN);河南科技学院高层次人才启动项目(2014020)

摘　　要：为了解鸡内源性抗菌肽NK-lysin的基本信息,试验选择鸡NK-lysin的氨基酸序列通过软件对NK-lysin的生物信息学进行了分析。结果表明:NK-lysin的等电点为5.87,分子质量为15 231.3 u,为稳定蛋白和亲水蛋白,定位于细胞质内,有1个跨膜区和1个信号肽,没有糖基化位点,但有2个磷酸化位点,二级结构由α螺旋、延伸链、β折叠和无规则卷曲组成,存在多个抗原表位。说明鸡抗菌肽NK-lysin可作用于细胞膜或某些生物分子,使菌体死亡。To know the basic information of chicken endogenous antimicrobial peptide NK - lysin, the amino acid sequence of chicken NK - lysin was selected to analyze the bioinformatics of NK - lysin by softwares. The results showed that the isoelectric point of NK - lysin was 5.87, and the molecular weight was 15 231.3 u. Chicken NK- lysin was a stabe and hydrophilic protein, and located in cytoplasm. Chicken NK- lysin has one transmembrane domain and one signal peptide, and had two phosphorylation sites without any glycosylation sites. Its secondary structure consisted of α- helix, extended strand, β - turn and random coil, and there were several antigenic epitopes. The results indicate that chicken NK - lysin can be applied to the cell membrane or some biological molecules to make the death of bacteria.

关 键 词：鸡 抗菌肽 NK—lysin 生物信息学 二级结构 抗原表位 

分 类 号：S852.4[农业科学—基础兽医学]