电针结合天麻素增加局灶性脑缺血大鼠额叶皮质生长相关蛋白-43以及突触素的免疫组化表达  

Electroacupuncture Combined Gastrodin Increased Immunohistochemical Expression of Focal Cerebral Ischemia in Rat Frontal Cortex GAP-43 and SYN

作　　者：陈鹏[1] 张大雁[1] 吴思苇 王圣宽 吴锋[2] 李怀斌[2] 

机构地区：[1]皖南医学院临床医学院 [2]皖南医学院解剖学教研室,安徽省芜湖市241002

出　　处：《医学理论与实践》2017年第1期1-3,共3页The Journal of Medical Theory and Practice

基　　金：国家级及安徽省大学生创新训练计划项目(201410368017,AH201410368017);皖南医学院重点科研项目培育基金(WK2014Z07);安徽省高校省级自然科学项目(WK2015A227)

摘　　要：目的:验证电针结合天麻素对局灶性脑缺血大鼠额叶皮质生长相关蛋白-43(Growth-associated protein,GAP-43)和突触素(Synaptophysin,SYN)表达是否有协同增效作用。方法:SD大鼠40只随机分为假手术组、模型组、电针组、天麻素组和电针结合天麻素组。采用线栓法阻塞大鼠右侧大脑中动脉复制局灶性脑缺血模型。造模成功后,按分组分别及同时给予电针刺激左侧曲池与合谷穴,天麻素注射液腹腔注射治疗。免疫组织化学SABC法检测额叶皮质GAP-43和SYN的表达。结果:与假手术组比较,模型组大鼠缺血侧额叶皮质GAP-43免疫反应阳性产物表达增加(P<0.05),SYN免疫反应阳性产物表达减少(P<0.05);与模型组比较,电针组、天麻素组和电针+天麻素组大鼠缺血侧额叶皮质GAP-43和SYN免疫反应阳性产物表达均显著增加(P<0.05);电针结合天麻素组GAP-43和SYN免疫反应阳性产物表达较电针组、天麻素组增加(P<0.05)。结论:电针结合天麻素对缺血侧额叶皮质GAP-43和SYN的表达具有协同增效作用,可能使神经元间联系结构增加,有利于脑缺血后神经功能的修复。Objective：To validate whether there is synergy effect of electroacupuncture（EA）combined with gastrodine on the expression of（Growth-associated protein,GAP-43）and（Synaptophysin,SYN）in frontal cortex in focal cerebral ischemia（FCI）rats.Methods：Forty SD rats were randomly divided into sham operation,model,EA,EA＋gastrodin groups.FCI rats were induced by middle cerebral artery occlusion with thread embolus.EA was applied to left side＂Quchi＂（LI 11）and＂Hegu＂（LI 4）after MCAO,gastrodin at 10mg/kg was given to rats by intraperitoneal.The expressions of GAP-43 and SYN in frontal cortex detected by immunohistochemical.Results：Compared with sham operation group,the expression of GAP-43 products of immune response increased（P〈0.05）,the expression of SYN products of immune response decreased（P〈0.05）.Compared with model group,the expression of GAP-43 and SYN products of immune response increased（P〈0.05）,expression levels in the EA＋gastrodin group increased significantly than the EA or gastrodin group（P〈0.05）.Conclusion：EA combined with gastrodin have synergistic effect on the expression of GAP-43 and SYN in the ischemic frontal cortex can promote the regeneration and repair of injured neurons.May contact increase the structure element between neurons,beneficial to the repair of neurological function after FCI.

关 键 词：脑缺血 电针 天麻素 生长相关蛋白-43 突触素 额叶皮质 

分 类 号：R285.5[医药卫生—中药学]