活动性肺结核与潜在感染者CD8＋、CD4＋记忆T细胞表达水平的比较  

Expression Level of CD8^＋ and CD4^＋ Memory T cells and Its Diagnostic Value in Patients with Active Tuberculosis and TB Latent Infection

作　　者：袁成良[1] 刘朝红[1] 张姚[1] 叶敏[1] 

机构地区：[1]四川省德阳市人民医院,四川德阳618000

出　　处：《医学临床研究》2016年第12期2383-2385,共3页Journal of Clinical Research

摘　　要：【目的】探讨CD8^＋、CD4^＋记忆T细胞表达水平在活动性肺结核与潜伏感染者中的表达差异及其意义。【方法】本院确诊的25例活动性肺结核患者（A组）、25例肺结核潜伏感染者（B组）、30例健康人群作为健康对照组（C组），检测各组外周血CD4^＋记忆T细胞、CD8^＋记忆T细胞的表达水平，并探讨其与高分辨率CT评分（HRCT）的相关性。【结果】B组的CD4^＋Tcm、CD8^＋Tcm水平显著高于A组、C组（P〈0．05），A组的CD4^＋Tcm、CD8^＋Tcm水平显著的高于C组（P〈0．05）。B组、A组的CD4^＋Tem、CD8^＋Tem水平显著的低于C组（P〈0．05），B组、A组的CD4^＋Tem、CD8^＋Tem水平相比差异无显著性（P〉0．05）。A组患者CD4^＋Tcm、CD8^＋Tcm水平与HRCT评分呈显著的负相关关系（P〈0．05）；A组患者CD4^＋Tem、CD8^＋Tem水平与HRCT评分无显著的相关系（P〉0．05）。[结论]CD4^＋、CD8^＋中心记忆性T细胞在活动性肺结核与潜伏感染者中水平差异显著，并且与肺结核患者病例损伤程度有关。[Objeetive]To investigate the expression and significance of CD8^＋ and CD4^＋ memory T cells in active pulmonary tuberculosis and TB latent infection. [Methods]Patients diagnosed with active tuberculosis and latent infection in our hospital were chosen as the subject. There were 25 cases of active pulmonary tuberculosis patients （TB group）, 25 cases of latent tuberculosis infection （latent infection group）, and 30 cases of healthy people （healthy group）. CD4＋ and CD8＋ memory T cells in peripheral blood were detected by flow cytometry. The results were compared with patients＇ high-resolution CT scores （HRCT） to analyze the correlation between the two. [Results]The level of CD4＋ Tcm and CD8＋ Tcm in the latent infection group was higher than that of the active pulmonary TB group and healthy group; the difference was statistically significant （ ） P 〈0.05）. Also, the expression of CD4＋ and CD8＋ Tcm in TB group was higher than that of the healthy group with statistical significance （ P 〈0.05）. On the other hand, the level of CD4＋Tem and CD8＋Tem in TB group and latent infection group was significantly lower than that in the healthy group; the difference was statistically significant （P 〈0.05）. However, the differential expression in CD4＋Tem and CD8＋ Tern between the patients with pulmonary tuberculosis and the patients with latent infection was not statistically significant （ P 〉0.05）. Furthermore, the level CD4＋ Tcm and CD8＋ Tcm in TB patients had significant negative correlation with HRCT score （ P 〈0.05）. However, in TB patients, the level of CD4＋Tem and CD8＋ Tern level had no significant correlation coefficient （ P 〉0.05） with HRCT score. [Conclusion]Levels of Central memory T cells of CD4＋ Tom and CD8＋ Tcm in the active and latent tuberculosis infection patients were significantly increased, which may reflect the degree of injury of pulmonary tuberculosis.

关 键 词：结核 肺/免疫学 CD8阳性T淋巴细胞 CD4阳性T淋巴细胞 

分 类 号：R521[医药卫生—内科学]