枸杞叶片RNA提取方法优化  

Optimizing of Different RNA Isolation Methods for LyciumLinn Leaves

作　　者：付海辉[1,2] 张波 程慧[1,2,3] 李彦龙 曹有龙 周军[1,2] 

机构地区：[1]云南省高校林木遗传改良与繁育重点实验室,云南昆明650224 [2]西南林业大学林学院,云南昆明650224 [3]国家枸杞工程技术研究中心,宁夏银川750002

出　　处：《北方园艺》2017年第1期114-117,共4页Northern Horticulture

基　　金：云南省林学一流学科建设经费资助项目;云南省高校林木遗传改良与繁育重点实验室开放基金资助项目;国家林木(含竹藤花卉)种质资源平台资助项目(2003DKA21003)

摘　　要：以"宁杞1号"枸杞叶片为试材,采用优化的CTAB法、异硫氰酸胍法、Trizol法以及SDS法提取叶片RNA,以期筛选出适合枸杞叶片的总RNA的提取方法。结果表明:常规方法不能提取到完整的RNA,且条带不清晰、杂质多。经优化后,异硫氰酸胍法较为理想,并且可以用于RT-PCR试验。The performances of four extraction methods,including CTAB,guanidinium isothiocyanate,Trizol and SDS were compared to select the suitable method for total RNA extraction fromLyciumLinn leaves.The results showed that RNA could not be extracted perfectly by original methods and RNA non-clearness as well as impurity,but the optimizing guanidinium isothiocyanate method was perfect and total RNA could be used for RTPCR.

关 键 词：枸杞 叶片 RNA 改良异硫氰酸胍法 

分 类 号：S567.19[农业科学—中草药栽培]