枸杞种质遗传多样性SCoT分析  

Genetic Diversity of Wolfberry Germplasm Based on SCoT Markers

作　　者：杨辉[1] 周旋 王学琴[1] 康磊[1] 梅杰[1] 杨剑[1] 

机构地区：[1]宁夏科技发展战略和信息研究所,宁夏银川750001 [2]国家枸杞工程技术研究中心,宁夏银川750002

出　　处：《北方园艺》2017年第2期95-98,共4页Northern Horticulture

基　　金：国家科技基础条件建设资助项目(2015DT01)

摘　　要：以17份枸杞种质为试材,采用SCoT标记法,分析了17份枸杞种质资源的遗传多样性,以期为枸杞种质资源的深度开发与利用提供参考。结果表明:从42条引物中筛选出7条重复性好、条带清晰的引物进行PCR扩增,共扩增出32条条带,其中30条条带具有多态性,多态性比率为93.47%;UPGMA聚类分析显示,17份枸杞种质间遗传相似系数在0.30~1.00,平均相似系数为0.80,在遗传相似系数为0.72时,可将17份枸杞资源分成四大类。表明采用SCoT标记法能够揭示枸杞种质间的遗传多样性。Seventeen wolfberry germplasm resources were used as test materials,by using SCoT markers,the genetic diversity of 17 wolfberry germplasm resources was studied to provide reference for the further development and utilization of wolfberry germplasm resources.The results indicated that selected 7reproducible and clear bands from 42 primers were amplified by PCR,a total of 32 bands were amplified,including 30 polymorphic bands,the polymorphic rate was 93.47%;the UPGMA cluster analysis showed that 17 genetic similarity coefficient of wolfberry germplasms was from 0.30 to 1.00,the average of the similarity coefficient was 0.80.When the genetic similarity coefficient was 0.72,the test materials could be divided into four categories.The genetic diversity of wolfberry germplasm could be revealed by SCoT markers.

关 键 词：枸杞 SCoT 遗传多样性 

分 类 号：S567.1[农业科学—中草药栽培]