MAS育种改良籼稻恢复系‘E32’及其杂交种的稻瘟病抗性  

Improving Blast Resistance of Rice Restorer ‘E32' and Its Hybrid through Molecular Marker-assisted Selection

作　　者：邹俊锋 李永聪[1] 刘雄伦[1,2,3,4] 刘金灵[1,2,3,4] 陈海龙[1] 杨丰宇[1] 黄俊[1] 廖花[1] 

机构地区：[1]湖南农业大学农学院,长沙410128 [2]水稻油菜抗病育种湖南省重点实验室,长沙410128 [3]作物基因工程湖南省重点实验室,长沙410128 [4]南方粮油作物协同创新中心,湖南长沙410128

出　　处：《作物研究》2017年第1期11-14,24,共5页Crop Research

基　　金：国家转基因生物新品种培育重大专项(2016ZX08001-002);湖南省科技重大专项(2015NK1001)

摘　　要：利用已克隆的广谱抗稻瘟病基因Pi9的DNA序列,设计功能分子标记Ins2-3,通过标记基因型辅助选择和连续回交育种实践,定向改良籼稻恢复系E32及其两系杂交种培矮64S/E32的稻瘟病抗性。获得以下结果:Ins2-3是一个高效共显性DNA标记,在Pi9供体亲本75-1-127和受体亲本E32基因组中分别扩增出一条793bp和2 kb的稳定条带,Ins2-3的辅助选择效率达100%;利用Ins2-3开展分子标记辅助选择育种,通过连续多代回交自交,育成了苗瘟和穗瘟抗性均显著提高的新恢复系E32-Pi9,其遗传背景与受体亲本E32一致;用E32-Pi9配制的两系杂交种培矮64S/E32-Pi9的稻瘟病抗性,比对照培矮64S/E32显著提高,而主要农艺性状及杂种优势表现非常相似。To improve blast resistance of the indica rice restorer E32 and its hybrid,a molecular marker,Ins2 -3,was de-veloped based on the DNA sequence of the cloned broad -spectrum blast resistance gene Pi9,and marker -assisted selec-tion breeding practice was implemented in this study.The main results were as follows：Ins2 -3 was a co -dominant mark-er,showing stable polymorphism between the Pi9 donor 75 -1 -127 and the receptor E32 by producing a 793bp and a 2kb PCR band respectively,and the phenotype selection efficiency by marker genotype reached 100%.A new restorer line E32-Pi9 and its hybrid Peiai64s/E32 -Pi9,with high -level seedling and spike blast resistance and unchanged genetic background,were bred.

关 键 词：水稻 稻瘟病抗性改良 Pi9 基因 分子标记辅助选择 

分 类 号：S511.034[农业科学—作物学]