来氟米特对免疫性肝损伤大鼠Kupffer细胞TRAIL表达的影响  

Effect of Leflunomide on TRAIL expression of KC cytokine from immunological liver injury rats in vivo

作　　者：张茜[1] 彭磊[2] 

机构地区：[1]安徽医科大学第二附属医院药剂科,合肥230601 [2]安徽医科大学药学院,合肥230032

出　　处：《安徽医学》2016年第12期1471-1473,共3页Anhui Medical Journal

基　　金：安徽医科大学校科研基金项目(项目编号:2012xkj075)

摘　　要：目的观察来氟米特(Lef)对免疫性肝损伤大鼠的保护作用及对Kupffer细胞(KC)中肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体表达的影响,探讨Lef防治肝损伤的作用及机制。方法雄性SD大鼠24只,随机分为正常组、模型组和Lef组,每组8只。模型组及Lef组采用卡介苗(BCG)+脂多糖(LPS)诱导大鼠免疫性肝损伤模型,Lef组再采用lef干预,考察Lef对大鼠免疫性肝损伤的保护作用;体外分离培养KC,观察Lef对KC分泌细胞因子TRAIL及其受体TRAIL-R的影响。结果 Lef可明显改善免疫性肝损伤大鼠肝细胞损伤、肝细胞变性坏死减轻,视野内凋亡小体变少。Lef能显著降低KC分泌的TRAIL、TRAIL-R1、TRAIL-R2表达,与模型组相比明显降低,差异有统计学意义(P<0.05)。结论 Lef对BCG+LPS诱导的免疫性肝损伤有保护作用,其机制可能与抑制TRAIL信号传导通路有关。Objective To investigate the role of Kupffer cells（KC） on regulation of apoptosis of liver cell（HC） and explore the molecular mechanism of Lef on prevention and treatment of immunological liver injury. Methods Rat model of immunological liver injury was established by combining gavage administration with bacillus Calmette Guerin（BCG） and lipopolysaccharides（LPS）,HC and KC were separated by the liver in situ perfusion method,and were cultured in vitro. Secretion of TRAIL and TRAIL-R of activated KC and HC were detected in each group. Results Lef reduced the cytokines TRAIL of KC secretion significantly and the expression of TRAIL-R in HC,KC on immune liver injury of rats,and liver cell apoptosis pathology changes of immunological liver injury in rats could be obviouslyimproved.Conclusion Lef can protect immune hepatic injury induced by BCG ＋ LPS by inhibiting TRAIL＇s expression,and the associated mechanism may be related to the inhibition of TRAIL signal transduction pathway.

关 键 词：来氟米特 免疫性肝损伤 肿瘤坏死因子相关凋亡诱导配体 肿瘤坏死因子相关凋亡诱导配体受体 

分 类 号：R965[医药卫生—药理学]