槲寄生化学成分研究  

Research on chemical constituents from branches and twigs with leaves of Viscum coloratum

作　　者：曹朵 韩畅[1] 高雯[1] 成亮[1] 杨培明[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200437

出　　处：《中草药》2016年第24期4313-4317,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的对槲寄生Viscum coloratum干燥带叶茎枝的化学成分进行研究。方法采用反复硅胶、Sephadex LH-20及ODS等柱色谱技术进行分离纯化,根据理化性质及波谱分析鉴定化合物的结构。槲寄生干燥带叶茎枝用95%乙醇提取浓缩后用水-氯仿萃取。其中水部位经D101大孔树脂,水-乙醇(100∶0→0∶100)梯度洗脱后得水和10%、30%、50%、70%、95%乙醇6个部位。结果在50%乙醇中分离得到9个化合物,分别鉴定为鼠李秦素-3-O-β-D-芹菜糖(1→2)-O-β-D-葡萄糖苷(1)、鼠李秦素(2)、鼠李秦素-3-O-β-D-葡萄糖苷(3)、鼠李秦素-3-O-β-D-(6″-乙酰)-O-β-D-葡萄糖苷(4)、高圣草素-7-O-β-O-葡萄糖苷(5)、高圣草素-7-O-β-D-芹菜糖基(1→2)-O-β-D-葡萄糖苷(6)、高圣草-7-O-β-D-葡萄糖基-4′-O-β-D-芹菜糖苷(7)、圣草酚-7-O-β-O-葡萄糖苷(8)、枫香槲寄生苷(9)。结论其中化合物1为新的黄酮苷类化合物,命名为槲寄生新苷IX,化合物9为首次从槲寄生中分离得到。Objective To study the chemical constituents from the dry stem leaves parts of Viscum coloratum. Methods The chemical constituents were separated and purified by various column chromatographies and HPLC. Their structures were determined on the basis of spectroscopic analyses（1H-NMR, 13C-NMR, 2D-NMR, and MS）. The dry stem leaves of V. coloratum were extracted by 95% ethanol, then concentrated and was extracted by water and chloroform. Then the water part was eluted by the gradient of water and ethanol（100∶0→0∶100）, obtaining the six parts of water, 10% ethanol, 30% ethanol, 50% ethanol, 70% ethanol, and 95% ethanol. Results Nine compounds were isolated from the 50% Et OH extract in the dry stem leaves parts of V. coloratum with the structures identified as rhamnazin-3-O-β-D-api（1→2）-O-β-D-glucoside（1）, rhamnazine（2）, rhamnazin-3-O-β-D-glucoside（3）, rhamnazin-3-O-β-D-（6″-acetyl）-O-β-D-glucoside（4）, homeriodictyol-7-O-β-D-glucoside（5）, homeriodictyol-7-O-β-D-api（1→2）-O-β-D-glucoside（6）, homeriodictyol-7-O-β-D-glucoside-4′-O-β-D-apioside（7）, homeriodictyol-7-O-β-D-glucosid（8）, and liquidamboside（9）. Conclusion Compound 1 is a new flavonoid glycoside, named as viscumneoside IX, and the compound 9 is isolated from the dry stem leaves parts of V. coloratumi first time.

关 键 词：槲寄生 鼠李秦素-3-O-β-D-芹菜糖(1→2)-O-β-D-葡萄糖苷 槲寄生新苷IX 鼠李秦素 高圣草素 枫香槲寄生苷 

分 类 号：R284.1[医药卫生—中药学]