东北岩高兰中查耳酮类化学成分研究  

Chalcones from Empetrum nigrum var. japonicum

作　　者：赵明[1] 黄淑蕾 王丹[1] 李军[1] 王金兰[1] 白丽明[1] 张树军[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《中草药》2016年第24期4318-4321,共4页Chinese Traditional and Herbal Drugs

基　　金：黑龙江省自然科学基金面上项目(B201209)

摘　　要：目的研究东北岩高兰Empetrum nigrum var.japonicum全草的查耳酮类化学成分。方法采用硅胶柱色谱和高效液相色谱等方法进行化学成分分离,并经波谱数据分析鉴定化合物的结构。用MTT法测试单体化合物对宫颈癌He La细胞的抑制活性。结果从东北岩高兰甲醇浸泡液正己烷萃取物中分离得到6个化合物,分别鉴定为2′,5′-二羟基-3′-甲氧基-4′,6′-二甲基二氢查耳酮(1)、4′,5′-二羟基-2′-甲氧基-3′,6′-二甲基二氢查耳酮(2)、2′,4′-二羟基二氢查耳酮(3)、2′,4′-二羟基查耳酮(4)、2′-甲氧基-4′-羟基二氢查耳酮(5)、2′,4′,β-三羟基二氢查耳酮(6)。结论化合物1、2为新化合物,命名为岩高兰素A和B,化合物5、6为首次从该植物中分离得到。化合物6具有一定的抗肿瘤活性。Objective To study the chemical constituents from Empetrum nigrum var. japonicum. Methods Compounds were isolated from n-hexane fraction in the industrial methanol extract from E. nigrum var. japonicum by silica gel column chromatography and HPLC. The structures of the chemical constituents were identified by analysis on their spectral data. All the isolated compounds were evaluated using relevant in vitro antitumor assay against He La cell lines. Results Six compounds were isolated from n-hexane fraction in the ethanol extract from E. nigrum var. japonicum and identified as 2′,5′-dihydroxy-3′-methoxy-4′,6′-dimethyl-dihydrochalcone（1）, 4′,5′-dihydroxy-2′-methoxy-3′,6′-dimethyl-dihydrochalcone（2）, 2′,4′-dihydroxy-dihydrochalcone（3）, 2′,4′-dihydroxy-chalcone（4）, 2′-methoxy-4′-hydroxy-dihydrochalcone（5）, and 2′,4′,β-trihydroxy-dihydrochalcone（6）. Conclusion Compounds 1 and 2 are new compounds, compounds 5 and 6 are obtained from the plants for the first time. Compound 6 exhibits the inhibitory activity.

关 键 词：东北岩高兰 查耳酮 二氢查耳酮 HeLa细胞 岩高兰素 2′ 5′-二羟基-3′-甲氧基-4′ 6′-二甲基二氢查耳酮 4′ 5′-二羟基-2′-甲氧基-3′ 6′-二甲基二氢查耳酮 

分 类 号：R284.1[医药卫生—中药学]