黄芪皂苷干预脾虚水湿不化证大鼠的代谢组学研究  

Intervention effects of Astragalus saponins on spleen deficiency syndrome rats based on metabonomic research

作　　者：刘阿娜[1] 崔宁[2] 赵文晓[2] 陈迩东 季旭明[2] 王世军[2] 蒋海强[3] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学中医学院,山东济南250355 [3]山东中医药大学实验中心,山东济南250355

出　　处：《中草药》2016年第24期4401-4407,共7页Chinese Traditional and Herbal Drugs

基　　金：国家重点基础研究发展计划("973"计划)课题(2013CB5318000)

摘　　要：目的研究黄芪皂苷组分干预脾虚水湿不化证大鼠血浆内源性代谢物的变化,探求黄芪皂苷组分对脾虚水湿不化证的干预作用。方法采用高效液相-飞行时间质谱仪检测大鼠血浆内源性代谢物信息,利用主成分分析和偏最小二乘判别分析研究对照组、模型组以及黄芪皂苷组之间的代谢差异,确定潜在生物标志物。结果对照组、模型组以及黄芪皂苷组代谢模式明显不同,共鉴定11个生物标志物,其通路主要涉及甘油磷脂代谢、鞘脂代谢、淀粉与蔗糖代谢和花生四烯酸代谢等。结论黄芪皂苷组分可能通过干预溶血磷脂酰胆碱导致的脂肪代谢紊乱、脂肪酸进入线粒体减少导致的能量供给不足、肠道功能失调等一系列代谢紊乱对脾虚水湿不化证起到治疗作用。Objective Effect of Astragalus saponins on the changes of endogenous metabolites in plasma of rats with spleen deficiency syndrome of dampness and the intervention function of Astragalus Radix saponins on the syndrome was studied. Methods High performance liquid phase of flight time mass spectrometry was used to detect the information of endogenous metabolites in rat plasma, principal component analysis and partial least-squares discriminate analysis were used to study the metabolic differences between normal group, model group, and astragalus saponins group to identify potential biomarkers. Results The metabolism pattern of normal group, model group, and Astragalus saponin group were significantly different, 11 biomarkers were identified and the pathway mainly involved glycerol phospholipids metabolism, sphingolipid metabolism, pentose glucuronic acid conversion, and peanut arachidonic acid metabolism. Conclusion The results of this study show that it is possible for Astragalus Radix saponins group to interfere with the spleen deficiency syndrome of dampness by regulating energy metabolism, lipid metabolism, and fatty acid metabolism to exert therapeutic effect.

关 键 词：黄芪皂苷 脾虚水湿不化证 代谢组学 高效液相色谱-飞行时间质谱 脂肪代谢 

分 类 号：R285.5[医药卫生—中药学]