狨猴腹泻重要病原菌的分离鉴定与药敏试验研究  

Study on Isolation and Identification of Marmoset Diarrhoea Pathogenic Bacteria Strain and Its Sensitivity Tests to Drug

作　　者：张占恒 高珂珂 李小慧[3] 林静[3] 洪莹莹[3] 金天明[3] 韩文瑜[1] 

机构地区：[1]吉林大学动物医学学院,长春130062 [2]天津医科大学实验动物科学部,天津300070 [3]天津农学院动物科学与动物医学学院,天津300384

出　　处：《天津农学院学报》2016年第4期5-8,共4页Journal of Tianjin Agricultural University

基　　金：天津市优秀科研创新团队资助项目"兽医生物技术"(TD12-5019);天津市优秀科技特派员项目"规模化猪场腹泻病的监测及净化技术研究"(16JCTPJC45000);天津市农业科技成果转化与推广项目"规模化猪场仔猪腹泻病的监测及防控技术推广"(201601380)

摘　　要：狨猴腹泻病是一种常见疾病,临床症状主要表现为腹泻、消瘦、精神不振等。对临床发现的天津市某动物中心腹泻狨猴,采集粪便进行细菌分离培养、显微镜镜检、生化试验并进行16S r RNA片段扩增等,鉴定出导致该猴群腹泻的病原菌主要是大肠杆菌和沙门菌。药敏试验显示大肠杆菌对庆大霉素高度敏感,沙门菌对庆大霉素和氨苄西林高度敏感,为该病的治疗提供了依据。Diarrhoea is a kind of marmoset disease which mainly caused diarrhea, weight loss and spirit depressed. In this paper, the stool samples were collected from marmoset in an animal center in Tianjin. Then the samples were used for a series of laboratory diagnosis, such as bacterial isolation and culture, microscopic examination, biochemical tests and PCR detection of 16s rRNA to identified the isolated strains. The results showed that the isolated strains were Escherichia coli and Salmonella. Drug sensitive test was examined, and the results showed that the isolated Escherichia coli strain is highly sensitive to gentamicin, the isolated Salmonella strain is highly sensitive to gentamicin and ampicillin.

关 键 词：狨猴 腹泻 病原菌 分离鉴定 药敏试验 

分 类 号：S852.61[农业科学—基础兽医学]