盐胁迫下耐盐苜蓿松散型愈伤组织生理生化特性变化  

The Physiological and Biochemical Characteristics Changes of Loose Salt-tolerant Callus of Alfalfa under Salt Stress

作　　者：杨静慧[1] 吴志瑜[1] 刘艳军[1] 秦艳筠 李冰 黄唯子 

机构地区：[1]天津农学院园艺园林学院,天津300384 [2]天狮学院工商管理学院,天津301700

出　　处：《天津农学院学报》2016年第4期38-42,共5页Journal of Tianjin Agricultural University

基　　金：天津市农业科技成果转化与推广项目“彩叶树优良品种繁殖、栽培技术示范与推广”(201502100);天津市科技成果转化及产业化推进计划项目“优质彩叶北美海棠新品种种苗的规模化繁育”(14ZXNZNC0040)

摘　　要：为了解盐胁迫下耐盐突变苜蓿的生理生化变化,以青睐苜蓿耐盐突变体的松散型愈伤组织和青睐苜蓿松散型愈伤组织为试材,用2%Na Cl溶液浸泡1 h,分析了该组织在盐胁迫下细胞中的可溶性糖、丙二醛、可溶性蛋白、游离脯氨酸等的含量,以及细胞膜透性、离子浓度和SOD、POD酶活性。结果显示:盐胁迫下耐盐松散型愈伤比对照组织的可溶性糖含量、POD酶活性、游离脯氨酸含量、K^+/Na^+分别高出0.757倍、0.266倍、6.525倍、0.700倍,SOD酶活性、丙二醛含量、细胞膜透性、Na^+、K^+浓度分别低1.807倍、0.878倍、0.553倍、2.903倍、1.296倍。所以,耐盐松散型愈伤组织在盐胁迫下表现出可溶性糖含量、POD活性、游离脯氨酸含量、K^+/Na^+增加,丙二醛含量、细胞膜透性、Na^+浓度降低,可溶性蛋白无明显变化。试验结果表明苜蓿耐盐突变愈伤组织具有更强的耐盐性。In order to understand the physiological and biochemical changes of alfalfa salt resistant mutant, the content of soluble sugar, malondialdehyde, soluble protein, free proline and the cell membrane permeability, ionic concentration and activity of SOD ＆ POD enzyme were studied with loose salt-tolerant and ordinary callus of alfalfa and under 2% salt stress. The results showed that the soluble sugar, POD enzyme activity, free proline and K^＋/Na^＋ of loose salt-tolerant callus was respectively 0.757, 0.266, 6.525 and 0.700 times higher than control group. However, the SOD enzyme activity, malondialdehyde, cell membrane permeability, and Na^＋ ＆ K^＋ was 1.807, 0.878, 0.553, 2.903 and 1.296 times lower than control group. In conclusion, Loose salt-tolerant callus increased in soluble sugar, POD activity, free proline and the ratio of K^＋/Na^＋ under salt stress, while decreased in malondialdehyde cell membrane permeability and Na^＋, furthermore there was no significant change in soluble protein. In short, alfalfa mutations salt-tolerant callus have more resistance.

关 键 词：苜蓿 松散型 愈伤组织 盐胁迫 生理生化 

分 类 号：S541.9[农业科学—作物学]