基于氯化血红素复合材料的过氧亚硝酸阴离子电化学传感器的构建  

Construction of Hemin Composite Based Electrochemical Sensor for Detection of Peroxynitrite

作　　者：任聚杰[1] 于聪聪[1] 崔敏[1] 李亚清[1] 武聪[1] 籍雪平[2] 

机构地区：[1]河北科技大学理学院,石家庄050018 [2]河北医科大学基础医学院,石家庄050017

出　　处：《分析化学》2017年第1期104-110,共7页Chinese Journal of Analytical Chemistry

基　　金：河北省自然科学基金(No.B2016206150);科技部中国-罗马尼亚国际合作项目(No.41-19)资助

摘　　要：构建了一种基于氯化血红素/金纳米粒子/聚三聚氰胺/多壁碳纳米管复合材料修饰玻碳电极的过氧亚硝酸阴离子电化学传感器,并成功用于过氧亚硝酸阴离子的检测。采用循环伏安法和电流-时间曲线考察了过氧亚硝酸阴离子在传感器上的电化学行为,并对传感器的制备条件及过氧亚硝酸阴离子的检测条件进行了优化。结果表明,碳纳米管滴涂量为5μL,金沉积时间为20 s,工作电位为0.8 V时,所制传感器对过氧亚硝酸阴离子的响应最大。在优化实验条件下,此传感器检测过氧亚硝酸阴离子的线性范围为1.0×10!5~3.5×10!4mol/L和3.5×10!4~1.1×10!3mol/L,灵敏度为0.13 A/(mol/L),检出限为1.2×10!7mol/L(S/N=3)。A peroxynitrite electrochemical sensor based on hemin/gold nanoparticles/poly（melamine）/multiwalled carbon nanotube composite modifying glassy carbon electrode was successfully constructed for the detection of peroxynitrite.The electrochemical behavior of peroxynitrite on the sensor was investigated by cyclic voltammetry and amperometric i-t curve,and the conditions for preparation of the sensor and detection of peroxynitrite were optimized.The results showed that the amperometric response of the sensor for peroxynitrite reached the maximum when the amount of carbon nanotubes was 5 μL,the deposition time of gold was 20 s,and the working potential was 0.8 V.Under the optimized experimental conditions,the sensor could detect peroxynitrite in a linear range from 1.0 × 10^-5mol/L to 3.5 × 10^-4mol/L and from 3.5 × 10^-4mol/L to1.1 ×10^-3mol/L,with a sensitivity of 0.13 A/（mol/L） and a detection limit of 1.2 ×10^-7mol/L（S/N = 3）.

关 键 词：过氧亚硝酸阴离子 氯化血红素 聚三聚氰胺 金纳米粒子 碳纳米管 电化学传感器 

分 类 号：O657.1[理学—分析化学]