西洋参茎叶皂苷对PC12细胞氧糖剥夺损伤的保护作用  

Protective effect of Panax quinquefolium saponins from steams and leaves on PC12 cell injured by oxygen-glucose deprivation

作　　者：张婷婷[1] 任鹏宇[1] 刘嘉祺[1] 翟凤国[1] 关利新[1] ZHANG Ting -ting etal(Department of pharmacology, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, China)

机构地区：[1]牡丹江医学院药理教研室,黑龙江牡丹江157011

出　　处：《牡丹江医学院学报》2017年第1期28-30,80,共4页Journal of Mudanjiang Medical University

基　　金：国家自然科学基金课题(81374007;81641125);黑龙江省自然科学基金课题(H201379);黑牡丹江医学院研究生创新科研课题(2014YJSCX-14)

摘　　要：目的探讨西洋参茎叶皂苷对PC12细胞在氧糖剥夺损伤中的保护作用及可能机制。方法以缺氧缺糖诱导PC12细胞损伤模型(OGD)模拟脑缺血病理的改变,应用MTT法检测细胞存活率,分光光度计检测乳酸脱氢酶(LDH)释放量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)及一氧化氮(NO)含量,研究西洋参茎叶皂苷对该氧糖剥夺损伤模型的影响。结果与正常对照组比较,模型组PC12细胞在OGD损伤后细胞存活率和SOD活性显著下降(P<0.01),LDH释放量显著增加(P<0.01),MDA和NO水平均明显升高(P<0.01)。与模型组比较,西洋参茎叶皂苷高、中剂量组可明显提高细胞的存活率(P<0.01)和SOD活性(P<0.01),减少LDH释放(P<0.01),降低MDA和NO水平(P<0.01),差异具有统计学意义。结论西洋参茎叶皂苷对PC12细胞氧糖剥夺损伤具有保护作用,其保护机制可能与PQS稳定细胞膜,抗氧化损伤及清除自由基的作用有关。Objective To investigate the protective effect of Panax quinquefolium saponins from steams and leaves （PQS） on PC12 cell injured by oxygen - glucose deprivation（OGD） as well as the possible mechanisms. Methods A pheochromocytoma cell in- jury model was induced by OGD to simulate the cerebral isehemic changes. The protective effects of PQS were investigated in this mod- el. The MTT assay and the lactate dehydrogenase （LDH） release were used to evaluate the protective effects of PQS. Superoxide dis- mutase （SOD）, malondialdehyde （MDA） and nitric oxide （NO） were determined by speetrophotometry using commercial kits. Re- sults Compared with the control group, oxygen -glucose deprivation injury model group significantly reduce the cell survival rate and SOD activities（ P 〈 0.01 ） ,increased the LDH leakage rate（ P 〈 0.01 ）, enhanced the content of MDA and NO（ P 〈 0.01 ）. Treatment with PQS （200, 1 oo μg·mL^-1） significantly enhanced the cell viability and SOD activities （P 〈 0.01 ）, reduced LDH release （P 〈 0.01 ） , decreased the content of MDA and NO（ P 〈0.01 ）of the pheochromocytoma ceils compared with the OGD group. Conclusion PQS offers protective effect against OGD- induced injury in PC12 cells, and the possible mechanism may be associated with the im- provement of cell antioxidant capacity and scavenging free radical.

关 键 词：西洋参茎叶皂苷 氧糖剥夺损伤模型 神经保护 

分 类 号：R9[医药卫生—药学]