亚砷酸钠对SV-HUC-1细胞NRF2通路影响  

Effect of sodium arsenite on NRF2 pathway in SV-HUC-1 cells

作　　者：王惠惠[1] 陈锋[1] 朱佳玉[1] 席淑华[2] 孙贵范[3] 

机构地区：[1]中国医科大学公共卫生学院环境毒理学研究室,辽宁沈阳110122 [2]中国医科大学公共卫生学院地球化学性疾病研究室 [3]中国医科大学公共卫生学院环境与慢性病研究中心

出　　处：《中国公共卫生》2017年第1期95-97,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金青年项目(81502841);国家自然科学基金(81373023)

摘　　要：目的探讨亚砷酸钠(NaAsO_2)对人膀胱上皮细胞系SV-HUC-1细胞内核因子相关因子2(NRF2)活性的影响。方法时间效应实验以4μmol/L NaAsO_2分别处理SV-HUC-1细胞0(对照)、4、8、16、24 h;剂量效应实验设对照组和NaAsO_2处理组(1、2、4、8、10μmol/L);Western blot免疫印迹试验检测NRF2通路相关蛋白(NRF2、NQO1和HO1)表达水平。结果 4μmol/L NaAsO_2处理SV-HUC-1细胞4、8、16、24 h后,与对照组(0.68±0.03)比较,细胞内NRF2蛋白表达水平明显升高,16 h处理组NRF2蛋白表达最高(1.17±0.10),差异有统计学意义(P<0.05);随着染砷剂量增加,NRF2、NQO1和HO1蛋白表达水平增强,与对照组NRF2(0.86±0.05)、NQO1(0.68±0.02)、HO1(0.09±0.01)比较,4和8μmol/L NaAsO_2处理组细胞内NRF2、NQO1、HO1蛋白表达水平分别为(1.12±0.01)和(1.16±0.11)、(0.93±0.01)和(1.15±0.19)、(1.28±0.01)和(1.30±0.02),均明显升高,差异均有统计学意义(均P<0.05)。结论 NaAsO_2急性暴露能够活化NRF2通路,引起膀胱上皮细胞适应性抗氧化反应增强。Objective To investigate the effect of sodium arsenite on nuclear factor-erythroid 2-related factor 2 （NRF2） activity in human uroepithelial cells （ SV-HUC-1 ）. Methods The SV-HUV-1 cells were treated with 4 μmol/L sodium arsenite （NaAsO2 ） for 0 （control group） ,4,8,16, and 24 hours for time-effect evaluation and the treatments of 16 hours with NaAsO2 of 1,2,4,8 and 10 μmol/L were adopted for dose-response assessment. NRF2 pathway related proteins （ NRF2 ,NAD（P） H-quinone oxidoreductase 1 [ NQO1 ] and heme oxygenase-1 [ HO1] ） were detected with Western blot. Results The NRF2 protein levels were significantly increased in the SV-HUC-1 cells after exposed to 4μmol/L NaAsO2 for 4,8,16, and 24 hours compared to that in the control cells （0. 68 ± 0. 03 ） and the maximum NRF2 protein level （ 1.17 ± 0. 10） was observed in the cells treated for 16 hours （ P 〈 0. 05 ）. The protein levels of NRF2, NQO1 and HO1 increased in dose-response manner when treated with various dosages of NaAsO2 for 16 hours. Compared to the protein levels of NRF2 （0. 86 ± 0.05 ）, NQO1 （0. 68 ± 0.02）, and HO1 （0.09 ± 0. 01 ） in the control group, the levels of NRF2,NQOI,and HO1 increased to 1.12 ±0.01,1.16 ±0.11 and 0.93 ±0.01 and 1.15±0.19,1.28 ±0.01 and 1.30 ± 0. 02 in the cells treated with 4 and 8 μmol/L NaAsO2, respectively （ all P 〈 0.05 ）. Conclusion Acute NaAsO2 exposure can activate NRF2 pathway and induce adaptive antioxidant response in SV-HUC-1 ceils.

关 键 词：亚砷酸钠 SV-HUC-1细胞 核因子相关因子2(NRF2) 适应性抗氧化反应 

分 类 号：R114[医药卫生—卫生毒理学]