苯酚反硝化降解特征分析  

Degradation Characteristics Analysis of Phenol During Denitrification

作　　者：王孝维[1] 韩钰洁 岳秀萍[1] 

机构地区：[1]太原理工大学环境科学与工程学院,山西太原030024

出　　处：《中北大学学报（自然科学版）》2016年第6期620-624,658,共6页Journal of North University of China(Natural Science Edition)

基　　金：国家自然科学基金资助项目(51378330)

摘　　要：以苯酚作为碳源,NaNO3为反硝化氮源,接种成熟反硝化污泥,在厌氧发酵罐连续运行,定期取样分析苯酚、NO-x-N浓度变化情况,确定反硝化降解动力学特征;在线监测反应过程中pH和ORP变化规律;对反硝化污泥进行PCR-DGGE测定,分析系统微生物特征.在C/N比为4时,苯酚去除率83.3%,降解速率6.45mg/(g VSS·d),NO-x-N降解速率3.85mg/(g VSS·d).反硝化过程出现NO-2-N积累,最大积累率43.1%.pH和ORP曲线出现明显的"NO-2-N积累量最大点"和"反硝化真正结束点",可用来指示反硝化进程.苯酚反硝化降解细菌菌纲主要以Alphaproteobacteria,Gammaprotobacteria,Betaproteobacteria变形菌门和Bacteroidetes拟杆菌门为主;系统中检出了Altererythrobacter sp.,Bacteroides sp.,Pseudomonas baetica,Thermomonas sp.等苯酚、硝酸盐有效降解菌.Vaccinating mature denitrifying sludge into the anaerobic fermentation tank,it was operated continuously with phenol as carbon source and NaNO3 for denitrifying nitrogen source.The degrading dynamics characteristic of denitrification was carried out by measuring concentration changes of phenol and NO-x-N regularly.pH and ORP were monitored online in the process of reaction.Microorganism of denitrifying sludge was analyzed by PCR-DGGE.Results show that the C/N ratio was 4,the removal rate of phenol was 83.3%.The degradation rate of phenol was 6.45mg/（g VSS·d）,and the degradation rate of NO-x-N was 3.85mg/（g VSS·d）.NO-2-N accumulation appeared significantly,the maximum accumulation rate was 43.1%.The changes of pH and ORP could be used to reflect the denitrification process,and indicate＂the most points of NO-2-N accumulation＂and ＂the real end points of denitrification＂well.The classes of bacteria were mainly Proteobacteria and Bacteroidetes.Altererythrobacter sp.,Bacteroides sp.,Pseudomonas baetica,Thermomonas sp.etc detected in device were valid degrading bacteria for phenols and nitrates.

关 键 词：苯酚 反硝化 亚硝酸盐积累 氧化还原电位 菌群结构 

分 类 号：X703[环境科学与工程—环境工程]