4株海洋红球菌产类胡萝卜素分析及其全基因组测序  

Analysis on caroteniod producing of four marine Rhodococcus and its genome sequence determining

作　　者：张帝[1,2] 张宁[2] 陈吉刚[2] 杨季芳[2] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]浙江万里学院生物与环境学院,浙江宁波315100

出　　处：《海洋学研究》2016年第4期78-83,共6页Journal of Marine Sciences

基　　金：国家海洋公益性行业科研专项项目资助(201405015-5);宁波市科技公关重大项目资助(2012C10038);浙江省重中之重学科学生创新计划项目资助(CX2015007)

摘　　要：以源于东太平洋海水的4株红球菌分离菌Rhodococcussp.EPR-134、Rhodococcus sp.EPR-147、Rhodococcus sp.EPR-157和Rhodococcus sp.EPR-279为研究对象,开展了菌株的色素提取和色素全波长扫描,并基于全基因组测序分析了4株细菌类胡萝卜素代谢通路中的相关基因。色素全波长扫描结果显示,菌株EPR-134不具备类胡萝卜素产生能力,而其它3株红球菌能够产生类胡萝卜素,且所产类胡萝卜素组分不同。基因组分析表明,4个细菌基因组中存在较为完整的促使类胡萝卜素形成的基因簇。对菌株参与番茄红素形成的3个关键基因crt E、crt B和crt I的氨基酸序列同源性两两比对分析表明,菌株EPR-134 3个基因氨基酸序列与其它3个菌株相应基因氨基酸序列同源性最低,这可能是导致该菌株不产类胡萝卜素的关键原因。该研究结果为产类胡萝卜素红球菌的遗传改造,以及为产类胡萝卜素工程菌的构建奠定了前期基础。Rhodococcus sp.EPR-134,Rhodococcus sp.EPR-147,Rhodococcus sp.EPR-157 and Rhodococcus sp.EPR-279 were isolated from water samples of East Pacific Ocean,extraction of pigment and pigment full wavelength scan were carried out in this study.Based on the whole genome sequencing,the related genes in carotenoid metabolic pathways were analyzed.Pigment full wavelength scan results show that strain EPR-134 does not have a kind of caroteniod production ability,while other 3strains of Rhodococcus can produce carotenoids,and the carotenoid composition was different.Genome analysis show that there are more complete gene clusters in the 4bacterial genomes.crt E gene、crt B gene and crt I gene are very important in the metabolic pathway of caroteniod producing,and by analyzing amino acid sequence homology of these genes in different strains,it is find that amino acid sequences of these genes in the strain EPR-134 share the lowest sequence homology compared with other 3strains.This may be the key reason why strain EPR-134cant produce carotenoid.The results of this study may lay apreliminary foundation for genetic modification of the carotenoid producing Rhodococcus and construction of carotenoids producing engineering bacteria.

关 键 词：海洋红球菌 类胡萝卜素 基因组 代谢通路 

分 类 号：Q93[生物学—微生物学] Q78