模拟微重力环境及普通环境下小球藻的培养  

Culture of Chlorella sp. in Simulated Microgravity and General Conditions

作　　者：张志忍 王成成[1] 王为国[1] 汪铁林[1] 王存文[1] 

机构地区：[1]武汉工程大学化工与制药学院,湖北武汉430074

出　　处：《武汉工程大学学报》2016年第6期521-526,共6页Journal of Wuhan Institute of Technology

基　　金：湖北省自然科学基金(2015CFB580)

摘　　要：利用旋转细胞培养系统模拟微重力场,考察模拟微重力场及添加不同质量密度葡萄糖对小球藻(Chlorella sp.)生长情况的影响.研究结果表明,以BG-11为基础培养基,模拟微重力环境对小球藻生长有明显促进作用.当小球藻细胞接种密度为1.42×107个/毫升,培养时间为12 d时,普通环境下培养的小球藻最高细胞密度仅为5.80×107个/毫升,而在相同培养条件下模拟微重力环境下生长的小球藻细胞密度达到1.58×108个/毫升.在培养基中添加葡萄糖作为额外碳源后,微藻生长周期显著缩短,适宜小球藻生长的葡萄糖质量密度为10 g/L.当添加10 g/L葡萄糖,小球藻接种密度为5.76×107个/毫升,培养时间为5 d时,普通环境和模拟微重力环境下培养的小球藻的最高细胞密度分别可达3.85×108个/毫升和5.42×108个/毫升.The simulated microgravity condition was produced by the Rotary Cell Culture System to investigate effect of glucose mass density and simulated microgravity on the growth of Chlorella sp.. The results show that the simulated microgravity has an obvious promotion effect on the growth of microalgae. The algae cell density under general environment is 5.80× 10^7 cells/ mL when the initial cell density is 1.42× 10^7 cells/ mL and culture time is 12 days, meanwhile the algae cell density under simulated microgravity condition can reach 1.58×10^8 cells / mL. Fur/hermore, the microalgae growth circle is obviously shortened when glucose is added into the culture medium as carbon aource. The optimal glucose mass density for the Chlorella sp. growth is 10 g/L. When the initial cell density is 5.76× 10^7 cells/mL and culture time is 5 days, the maximum densities of microalgae cultured in simulated microgravity and general conditions reach 5.42× 10^8 cells/mL and 3.85×10^8 cells/mL, respectively.

关 键 词：模拟微重力 小球藻 葡萄糖 

分 类 号：Q949[生物学—植物学]