牵拉损伤对大鼠坐骨神经影响的实验研究  

Experimental research of effects of traction injury on the rat sciatic nerve

作　　者：童昌军[1] 魏优秀[1] 黄康[1] 

机构地区：[1]华中科技大学同济医学院附属梨园医院骨科,武汉430077

出　　处：《创伤外科杂志》2017年第1期35-38,共4页Journal of Traumatic Surgery

基　　金：华中科技大学自主创新研究基金资助项目(2013QN126)

摘　　要：目的探讨大鼠坐骨神经牵拉伤后MRI变化以及损伤过程中转化生长因子β1(transforming growth factorβ1,TGF-β1)的表达和Ⅰ、Ⅲ型胶原蛋白(COL-Ⅰ、Ⅲ)含量的变化。方法将50只成年雄性SD大鼠随机分为对照组(仅暴露坐骨神经)和牵拉组(大鼠右侧后腿坐骨神经牵拉伤),分别于术后3d,2、4、6、8周,随机取两组大鼠各5只,切取牵拉段神经行MRI、组织形态学、免疫组织化学的观察及反转录酶-聚合酶链式反应(RT-PCR)、Western Blot定量测定TGF-β1的表达和COL-Ⅰ、Ⅲ含量。结果牵拉损伤后MRI表现为神经增粗,T1、T2时间延长;牵拉组大鼠坐骨神经TGF-β1的表达和COL-Ⅰ、Ⅲ含量明显高于对照组,两者差异有统计学意义(P<0.05)。结论坐骨神经牵拉损伤后,周围神经发生纤维化病变,COL-Ⅰ、Ⅲ含量显著增加,TGF-β1表达有显著差异,提示TGF-β1在周围神经纤维化过程中具有一定的作用。Objective To investigate the change of MRI, transforming growth factor β1 （ TGF-β1 ） and col- lagen type Ⅰ ,Ⅲ （ COL- Ⅰ ,Ⅲ） after sciatic nerve traction injury. Methods Fifty adult male SD rats were randomly divided into group A and B. In group A （ the control group ） the sciatic nerve of rats was exposed only. In group B （ the traction injury group） the sciatic nerve of rats was tracted by smooth forceps to produce traction injury. Electron microscopy, immunochemistry, RT-PCR and Western Blot were performed to observe the morphological changes of the tracted nerve tissue and to determine the level of TGF-β1, COL- Ⅰ and COL- Ⅲ at 3 clays and 2,4,6, 8 weeks after the surgery, respectively. Results The injured nerve was characterized as thickened in size and increased T1 and T2 relaxation time. The expression of TGF-β1 in the traction injury group was significantly higher than that of the control group （ P 〈 0. 05 ）. In the meanwhile, the contents of COL- Ⅰ , COL-Ⅲ were positively correlated in a certain period （ P 〈 0. 05 ）. Conclusion Peripheral nerve fibrosis can be caused by nerve traction injury which induces increased expression of COL- Ⅰ , COL-Ⅲ in the nerve. TGF-β1 is significantly higher than that of the control group,which suggests that TGF-β1 plays an important role in the process of neural injury and fibrosis.

关 键 词：牵拉损伤 坐骨神经 MRI 转化生长因子 胶原蛋白 

分 类 号：R651.3[医药卫生—外科学]