基于UPLC-Q-TOF-MS/MS研究法半夏中甘草化学成分  

Analysis on Chemical Constituents of Glycyrrhizae Radix et Rhizoma in Pinelliae Rhizoma Praeparatum by UPLC-Q-TOF-MS/MS

作　　者：杨彬[1] 王媛[1] 田梦[1] 袁磊[1] 武媛媛[1] 李遇伯[1] 

机构地区：[1]天津中医药大学中药学院,天津300193

出　　处：《中国实验方剂学杂志》2017年第3期45-49,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB505300;2011CB505302)

摘　　要：目的:应用超高效液相色谱串联四级杆飞行时间质谱(UPLC-Q-TOF-MS/MS)对法半夏中所含甘草化学成分进行研究。方法:以法半夏水提液作为供试品溶液,采用Waters Acquity BEH C18色谱柱(2.1 mm×100 mm,1.7μm),乙腈-0.1%甲酸水为流动相进行二元梯度洗脱,流速0.25 m L·min-1,柱温35℃。质谱采用电喷雾离子源(ESI),负离子模式下扫描采集数据,根据准分子离子及二级碎片离子,并结合对照品及文献报道的质谱数据对法半夏中所含甘草化学成分进行鉴定。结果:从法半夏中鉴定出15个甘草化学成分。其中三萜皂苷类成分6个,黄酮类成分9个。结论:该研究通过UPLC-QTOF-MS/MS分析技术,明确了法半夏中含有的15种甘草化学成分,可以为阐明半夏经甘草炮制成为法半夏后功效发生变化的物质基础及其临床合理应用提供依据。Objective： To analyze the chemical components of Glycyrrhizae Radix et Rhizoma in Pinelliae Rhizoma Praeparatum（PRP） by using ultra performance liquid chromatography-quadrupole-time of flightmass spectrometry（UPLC-Q-TOF-MS/MS）. Method： The aqueous extracts of PRP were separated on Waters Acquity BEH C18column（2. 1 mm × 100 mm,1. 7 μm） with acetonitrile-0. 1% formic acid solution as mobile phase for gradient elution. The flow rate was 0. 25 m L·min-1and the column temperature was 35 ℃. Mass spectrometric data were obtained under electrospray ionization（ESI） negative mode and the constituents were identified according to the exact relative molecular weight and fragment ions reported in the references. Result：Fifteen constituents were identified from Glycyrrhizae Radix et Rhizoma in PRP,including 6 triterpenoid saponins and 9 flavonoids. Conclusion： Systematic research of the fifteen chemical constituents of Glycyrrhizae Radix et Rhizoma in PRP was performed firstly in this study,which could provide the basis for clarifying the pharmacodynamic material basis and rational clinical application of PRP.

关 键 词：超高效液相色谱串联四级杆飞行时间质谱 法半夏 甘草 化学成分 

分 类 号：R284.1[医药卫生—中药学]