三叶木通利尿活性部位筛选及其化学成分  

Screening of Diuretic Activity Fractions and Chemical Compositions in Rattan of Akebia trifoliate

作　　者：郭林新[1] 马养民[1] 李梦云[1] 乔珂[1] 王健[1] 

机构地区：[1]陕西科技大学化学与化工学院,教育部轻化工助剂化学与技术重点实验室,西安710021

出　　处：《中国实验方剂学杂志》2017年第3期66-70,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家公益性行业(林业)科研专项(200904004)

摘　　要：目的:筛选三叶木通利尿活性部位并对其化学成分进行研究。方法:采用大鼠代谢笼对三叶木通藤茎95%乙醇提取物的石油醚、乙酸乙酯、正丁醇萃取物和水溶部分的利尿作用进行测试。以硅胶柱色谱等方法对利尿作用显著的部位进行分离纯化,通过NMR,MS等方法并结合文献数据鉴定化合物结构。结果:正丁醇萃取物能显著增加大鼠尿量(P<0.01),其余各部位利尿作用不明显。在该部位分离得到9个化合物,分别鉴定为D-半乳糖(1),丁香酸-4-O-α-L-鼠李吡喃糖苷(2),常春藤皂苷元(3),肌醇(4),甘露醇(5),香草酸葡萄糖苷(6),丁香酸葡萄糖苷(7),常春藤皂苷元-3-O-α-L-阿拉伯吡喃糖苷(8)和β-谷甾醇-3-O-β-D-葡萄糖苷(9)。结论:三叶木通提取物的正丁醇萃取部位具有显著的利尿作用,其主要化学成分为苷类化合物。Objective： To screen the diuretic active fractions from rattan of Akebia trifoliate and study its chemical constituents. Method： The 95% ethanol extract of rattan of A. trifoliate,which were extracted with petroleum ether,ethyl acetate,butanol and water dissolving fractions,were investigated through metabolic cages experiments. The compounds of active fractions were isolated and purified by silica gel column chromatography and other methods,and their structures were identified by NMR and MS combined with literature data. Result： The butanol extract fraction can significantly increase the urine volume of rats（P 0. 01）,but the diuretic effect of other fractions was not obvious. Nine compounds were isolated from this diuresis fraction,and their structures were identified as D-galactose（1）,syringicacid-4-O-α-L-rhamnopyranoside（2）,hederagenin（3）,inositol（4）,mannitol（5）,vanillic acid-β-glucoside（6）,glucosyringic acid（7）,hederagenin-3-O-α-L-arabinopyranoside（8） and β-sitosterol-3-O-β-D-glycoside（9）. Conclusion： The butanol extract fraction had significant diuretic function,and glycosides were the main chemical composition of this fraction.

关 键 词：三叶木通 利尿作用 化学成分 分离鉴定 

分 类 号：R284.1[医药卫生—中药学]