杉寄生中酚性化合物的提取分离及含量测定  

Separation and Determination of Phenolsin Macrosolen cochinchinensis

作　　者：莫凯荃 许沛虎[1] 卢文杰[2,3] 黄周锋[2] 黄建猷[2] 陆国寿[2] 

机构地区：[1]武汉理工大学,武汉430070 [2]广西中医药研究院,南宁530022 [3]广西中药质量标准研究重点实验室,南宁530022

出　　处：《中国实验方剂学杂志》2017年第3期75-78,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西自然科学基金项目(2013GXNSFBA019204);广西卫生厅中医药科技专项(GZPT1230)

摘　　要：目的:建立杉寄生药材中鞣花酸和3,3'-二甲氧基鞣花酸的提取分离及含量测定方法。方法:采用溶剂提取、硅胶柱色谱和重结晶等提取分离手段得到酚性化合物鞣花酸和3,3'-二甲氧基鞣花酸;建立其HPLC含量测定方法:采用Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.2%磷酸水溶液(47∶53),流速0.8 m L·min-1,检测波长254 nm,柱温25℃。结果:鞣花酸在0.030 6~0.551 2μg与峰面积呈良好的线性关系(r=0.999 8),平均回收率为101.25%,RSD0.9%;3,3'-二甲氧基鞣花酸在0.018 9~0.339 5μg与其峰面积呈良好的线性关系(r=0.999 8),平均回收率101.34%,RSD1.5%。结论:鞣花酸和3,3'-二甲氧基鞣花酸为首次从杉寄生药材中分得,并建立了其HPLC含量测定方法,该方法简便、快速、具有较好的稳定性和重复性,可用于上述2种化合物含量同时测定。Objective： To establish the method for extraction,isolation and determination of ellagic acid and 3,3-di-O-methylellagic acid from Macrosolen cochinchinensis. Method： The ellagic acid and 3,3-di-Omethylellagic acid were isolated by solvent extraction,silica gel column chromatography and recrystallization techniques. Then HPLC content determination method was established： Eclipse XDB-C18 chromatographic column（4. 6 mm × 250 mm,5 μm） was used with methanol-0. 2% phosphoric acid（47 ∶ 53） as the mobile phase; the flow rate was 0. 8 m L·min-1; UV detection wavelength was set at 254 nm and the column temperature was 25 ℃.Result： Ellagic acid showed good linear relationship within 0. 030 6-0. 551 2 μg（r = 0. 999 8） and its average recovery rate was 101. 25%,RSD 0. 9%. 3,3-di-O-methylellagic acid showed good linear relationship within0. 018 9-0. 339 5 μg（r = 0. 999 8） and its average recovery rate was 101. 34%,RSD 1. 5%（n = 6）.Conclusion： The ellagic acid and 3,3-di-O-methylellagic acid were obtained from M. cochinchinensis for the first time,and the HPLC method was established for content determination. This method was simple,rapid and with great stability and repeatability,which could be used to determine the contents of above two components from M.cochinchinensis.

关 键 词：杉寄生 鞣花酸 3 3'-二甲氧基鞣花酸 提取分离 

分 类 号：R284.1[医药卫生—中药学]