新疆阿魏乙酸乙酯提取物对人结肠腺癌Caco-2细胞迁移、侵袭的影响及相关机制  

Ferula Sinkiangensis Ethyl Acetate Extract on Migration and Invasion of Human Colon Adenocarcinoma Cell Line Caco-2 and Relevant Mechanism

作　　者：雷亚江 刘柏里 罗福祥[1] 张海英[1,2] 

机构地区：[1]新疆医科大学,乌鲁木齐830011 [2]新疆医科大学附属中医医院,乌鲁木齐830000

出　　处：《中国实验方剂学杂志》2017年第3期110-115,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81260625)

摘　　要：目的:研究新疆阿魏乙酸乙酯提取物对人结肠腺癌Caco-2细胞迁移、侵袭的影响,并探讨其相关机制。方法:以Caco-2细胞为研究对象,用6.25,12.5 mg·L-1新疆阿魏乙酸乙酯提取物和顺铂分别作用于处于对数生长期的人结肠腺癌Caco-2细胞,并设空白组,用划痕愈合法观察0~48 h各组Caco-2细胞迁移情况,用transwell小室法计数72 h各组Caco-2细胞穿过基质膜个数,并计算出每个时间段Caco-2细胞迁移率和侵袭抑制率。采用荧光实时定量PCR法和蛋白免疫印迹法(Western blot)检测各组Caco-2细胞中磷酸酶(phosphatase and tensin homolog deleted on chromosome ten,PTEN),丝氨酸/苏氨酸蛋白激酶(serine/threonine,Akt),哺乳动物雷帕霉素靶蛋白(mammalina target of rapamgcin,m TOR)基因和蛋白的表达水平。结果:与空白组比较,新疆阿魏乙酸乙酯提取物和顺铂组在24,48 h细胞愈合程度变慢(P<0.05);72 h时穿过基质膜的细胞个数显著减少(P<0.01),各用药组PTEN基因表达水平明显升高,Akt,m TOR基因表达水平明显较低(P<0.05)。结论:新疆阿魏乙酸乙酯提取物可以显著抑制人结肠腺癌Caco-2细胞的迁移和侵袭,可能与提高PTEN基因表达水平有关。Objective： To study the effect of Ferulae Resina ethyl acetate extract on the migration and invasion of human colon adenocarcinoma cell line Caco-2 and its relevant mechanism. Method： With Caco-2 cells as the research object,6. 25,12. 5 mg·L-1of Ferulae Resina ethyl acetate extract and cisplatin were respectively applied in human colon adenocarcinoma Caco-2 cells in the logarithmic phase of growth. A blank control group was set up. Scratch healing method and transwell chamber were used to observe 0-48 h Caco-2 cells migration in each group and the number of Caco-2 cell passing through the matrix membrane in 72 h in each group,and calculate Caco-2 cell migration rate and invasion rate at each time point. Gene and protein expressions of phosphatase and tensin homolog（PTEN）,serine/threonine（Akt）,mammalina target of rapamgcin（m TOR）,and protein in Caco-2 cells were detected by Real-time PCR and Western blot. Result： Ferulae Resina and cisplatin groups showed slower cells healing degrees at 24 h and 48 h compared with the blank group（P 0. 05）,at 72 h,the number of cells passing through the matrix membrane was significantly less than that in blank group（P 0. 01）. In each dose group,PTEN gene expression levels were significantly higher,and Akt,m TOR gene expression levels were significantly lower（P 0. 05）. Conclusion： Ferulae Resina ethyl acetate extract can significantly inhibit the migration and invasion of human colon adenocarcinoma Caco-2 cells,which may be correlated with increase in PTEN gene expression.

关 键 词：新疆阿魏 CACO-2细胞 迁移 侵袭 磷酸酶 丝氨酸/苏氨酸蛋白激酶 哺乳动物雷帕霉素靶蛋白 

分 类 号：R285.5[医药卫生—中药学]