沙门菌去泛素化酶SseL对泛素链的水解特异性研究  

Research on hydrolysis specificity of Salmonella deubiquitinase SseL in hydrolysis of ubiquitin chains

作　　者：郑学明[1] 周海森 芮志莲 鞠爱萍[4] 胡红娟 孙溧鸿 王建强 傅行礼[1] 

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]江苏省南京市溧水区人民医院,江苏南京211200 [3]江苏省常州市溧阳人民医院,江苏常州213300 [4]广东省广州市花都区妇幼保健院检验科,广东广州510800 [5]江苏省常州市金坛第二人民医院,江苏常州213200

出　　处：《中国现代医学杂志》2017年第1期24-28,共5页China Journal of Modern Medicine

基　　金：江苏大学科研启动基金项目(No:14JDG043);江苏省重点研发计划(社会发展项目)(No:BE2016663);江苏省南京市社会发展科技计划项目(No:201303032);江苏省常州市社会发展科技计划-应用基础研究项目(No:CY20130026)

摘　　要：目的探讨沙门菌毒力效应蛋白Sse L对不同连接方式连接泛素链的水解特异性。方法克隆伤寒沙门菌Ty2菌株Sse L基因,融合表达纯化GST-Sse L蛋白并在体外研究其对Ub-AMC及各种泛素链的水解情况。结果 Sse L对Ub-AMC水解活性很低,对直链泛素基本没有活性;而对K48和K63连接的二泛素表现出很高的水解活性。结论沙门菌毒力效应蛋白Sse L具有特异性水解泛素异肽链活性,并且对K63多聚泛素链具有更高地水解活性。Objective To investigate the specificity of Salmonella virulence effector protein SseL in hy- drolysis of nbiqnitin （Ub） chains. Methods SseL gene was cloned from the Salmonella entedca serovar Typhi strain Ty2. GST-SseL was expressed and purified in vitro and its ability in hydrolysis of Ub-AMC and various ubiquitin chains was studied. Results SseL had only low hydrolytic activity to Ub-AMC and no hydrolytic activity to linear ubiquitin chains. Moreover, SseL showed higher hydrolytic activity to Lys63-linked diubiquitin （K63 diUb） than to Lys48-linked diubiquitin （K48 diUb）. Conclusions Salmonella effector protein SseL has higher hydrolytic specificity for isopeptide-linked ubiquitin and higher hydrolytic efficiency to Lys63-linked polyubiquitin.

关 键 词：沙门菌 效应蛋白 去泛素化酶 二泛素 Ub-AMC 

分 类 号：R378[医药卫生—病原生物学]