蜈蚣提取物对小鼠急性肝损伤的保护作用  

Protective effects of extract from Scolopendra subspinipesmutilans on mice with acute liver injury

作　　者：王程[1] 康四合 李娟[1] 薛珊[1] 陈科力[1] 

机构地区：[1]教育部中药资源与中药复方重点实验室&湖北中医药大学,湖北武汉430065

出　　处：《中国医院药学杂志》2017年第1期21-24,共4页Chinese Journal of Hospital Pharmacy

基　　金：重大新药创制国家科技重大专项"中药新药安全性检测技术与标准研究"(编号:2014ZX09304307001)

摘　　要：目的:研究蜈蚣水提取物和不同体积分数乙醇提取物对小鼠急性肝损伤的保护作用。方法:采用四氯化碳(CCl_4)制备小鼠急性肝损伤模型,测定血清中谷草转氨酶(AST),谷丙转氨酶(ALT)和肝脏中超氧化物歧化酶(SOD),丙二醛(MDA)水平;HE染色观察肝脏病理形态改变;RT-PCR检测小鼠肝脏中的肿瘤坏死因子α(TNF-α),白介素6(IL-6)mRNA表达水平。结果:与CCl_4模型组比较,蜈蚣水提物和30%乙醇提取物可以降低急性肝损伤小鼠ALT,AST和MDA水平,提高SOD水平;减轻小鼠肝脏病理损伤;降低TNF-α及IL-6的mRNA表达。其中水提物的效果更为显著。结论:蜈蚣水提物和30%乙醇提取物对小鼠急性肝损伤有一定的保护作用,其机制可能与抗脂质过氧化作用以及抑制TNF-α与IL-6的表达有关。OBJECTIVE To study the protective effects of water and ethanol extracts from Scolopendra subspinipesmutilans on mice with acute liver injury.METHODS Mouse models with acute liver injury were induced by carbon tetrachloride（CCl4）.ALT,AST in serum and SOD,MDA in liver were determined.The liver pathological morphological changes were detected by HE staining.RT-PCR was used to detect the mRNA expressions of TNF-αand IL-6.RESULTS Compared with model group,levels of ALT,AST and MDA were significantly decreased,and SOD level was increased in water extract group and 30%ethanol extract group;the histopathological damage of liver was reduced.The mRNA expressions of TNF-αand IL-6 were decreased.Water extract group showed superior effect.CONCLUSION Water extract and 30% ethanol extract from Scolopendra subspinipesmutilans show protective effects on mice with CCl4 induced acute liver injury.The mechanism is associated with antilipid peroxidation and inhibition of TNF-αand IL-6 expressions.

关 键 词：蜈蚣提取物 四氯化碳 肝损伤 

分 类 号：R931.74[医药卫生—生药学]