体外心脏震波治疗通过PI3K/Akt信号传导通路对人脐静脉内皮细胞增殖和凋亡的影响  

Effect of extracorporeal cardiac shock wave therapy on human umbilical vein endothelial cells proliferation and apoptosis through PI3K/Akt signaling pathway

作　　者：马一铭 李丽[1] 郭涛[1] 肖践明[1] 胡钊[1] 蔡红雁[1] 

机构地区：[1]昆明医科大学第一附属医院心内科,昆明650000

出　　处：《临床心血管病杂志》2017年第1期79-82,共4页Journal of Clinical Cardiology

基　　金：国家自然科学基金项目(No:81260027);云南省科技计划项目-省应用基础研究(昆医联合专项)(No:2014FZ023);云南省医学学科带头人培养项目(No:D-201622)

摘　　要：目的:研究体外心脏震波治疗(ESWT)对人脐静脉内皮细胞(HUVECs)增殖和凋亡的影响及作用机制。方法:体外培养的人脐静脉内皮细胞分成3组,即对照组、ESWT组和ESWT+LY294002组。CCK8比色法检测细胞增殖情况,Annexin V/PI流式细胞术检测细胞凋亡率,蛋白质印迹方法(Westen blot)检测Bax、Bcl-2、p-Akt和Akt蛋白的表达水平。结果:ESWT能明显提高HUVECs的增殖能力(P<0.001),抑制凋亡率(P<0.001),减少HUVECs内Bax蛋白表达水平,增加Bcl-2和p-Akt蛋白表达水平(均P<0.01),但这些作用可被PI3K特异性抑制剂LY294002削弱。结论:磷脂酰肌醇3激酶(PI3K)/丝/苏氨酸激酶(Akt)及其下游信号通路介导了ESWT促进HUVECs增殖及抑制凋亡作用。Objective：To investigate the effect of extracorporeal shock wave therapy（ESWT） on the proliferation and apoptosis in human umbilical vein endothelial cells（HUVECs） and to explore its underlying mechanism.Method：HUVECs cultured in vitro were divided into three groups：normal control group,ESWT group,and LY294002 plus ESWT.The proliferation of the cells were assayed by CCK-8 assay.The apoptosis rates were detected by flow cytometry.The expression of Bax,Bcl-2,p-Akt and Akt protein in the cells were assessed by Western blotting.Result：ESWT markedly promoted the proliferation of HUVECs（P0.001）,and decreased the apoptosis rate（P0.001）.ESWT also down-regulated the expression of Bax,and up-regulated the expression of Bcl-2 and p-Akt.However,the effects of ESWT on HUVECs were inhibited by phosphatidylinositol 3-kinase inhibitor LY294002.Conclusion：ESWT induces the proliferation and inhibits the apoptosis in HUVECs.PI3K/Akt and the related downstream targets are involved in this process.

关 键 词：体外心脏震波治疗 人脐静脉内皮细胞 增殖 凋亡 信号通路 

分 类 号：R541.3[医药卫生—心血管疾病]