QuEChERS/液相色谱-串联质谱法测定5种蔬菜中17种氨基甲酸酯类农药的基质效应研究  

Detection of Matrix Effects of 17 Carbamate Pesticides in 5 Vegetables by QuEChERS/LC-MS/MS Method

作　　者：许文娟[1] 王振刚[2] 丁葵英[1] 孙军[1] 郭礼强[1] 

机构地区：[1]潍坊出入境检验检疫局,山东潍坊261041 [2]潍坊医学院附属医院,山东潍坊261041

出　　处：《分析测试学报》2017年第1期54-60,共7页Journal of Instrumental Analysis

基　　金：质检总局科技计划项目(2016IK208);山东出入境检验检疫局项目(SK201420);潍坊市科学技术发展项目(2015ZJ1101)

摘　　要：建立了QuEChERS/液相色谱-串联质谱法测定5种蔬菜中17种氨基甲酸酯农药残留的方法,研究了该方法下姜、大葱、黄瓜、胡萝卜和白菜对17种农药的基质效应,并探讨了基质种类、基质浓度、农药浓度对基质效应的影响。结果表明:不同基质中的17种氨基甲酸酯农药在5~100μg/L浓度范围内线性关系良好,平均回收率为76.5%~111.8%,相对标准偏差(n=6)为2.5%~8.2%。5种常见蔬菜对17种氨基甲酸酯农药均存在不同程度的基质效应,且多数呈基质抑制效应,其中,基质种类、基质浓度和农药浓度均会影响基质效应强度。胡萝卜对硫双威和乙霉威、黄瓜对硫双威的基质效应均在0.8~1.1之间,日常检测中,可根据实际需求忽略其基质效应影响。但对于其他蔬菜中氨基甲酸酯类农药的检测,必须考虑基质效应的影响。A QuEChERS coupled with liquid chromatography-tandem mass spectrometric（LC-MS/MS） method was developed for the detection of 17 carbamate pesticides in five vegetables.The matrix effects in ginger,scallion,cucumber,carrot and cabbage,together with the influences of matrix type,matrix concentration and pesticide concentration on the matrix effects were discussed.The results showed that,the liner ranges of 17 pesticides in different vegetables ranged from 5 μg/L to 100 μg/L,and the recoveries ranged from 76.5% to 111.8% with relative standard deviations of 2.5%-8.2%.Different vegetables present different matrix effects for the responses of 17 pesticides in LC-MS/MS,mostly the matrix suppression effects,which were impacted by matrix type,matrix concentration and pesticide concentration.In addition,the matrix effects of thiodicarb and diethofencarb in carrot and thiodicarb in cucumber ranged from 0.8-1.1 despite of matrix concentrations and pesticide concentrations,suggesting the matrix effects of these circumstances advisably being ignored in routine testing.However,the matrix effects must be considered carefully during detection procedures of 17 carbamate pesticides in ginger,scallion,cucumber,carrot and cabbage.

关 键 词：QUECHERS 基质效应 液相色谱-串联质谱 蔬菜 氨基甲酸酯 农药残留 

分 类 号：O657.63[理学—分析化学] F767.2[理学—化学]