阿维菌素生产菌的常压室温等离子体诱变育种及培养基优化  

Atmospheric and room temperature plasma breeding and medium optimization of avermectin producing strains

作　　者：田萍萍 曹鹏[1] 常传友 胡栋[1] 张健[1] 高强[1] 

机构地区：[1]工业发酵微生物教育部重点实验室天津科技大学生物工程学院,天津300457

出　　处：《微生物学通报》2017年第1期150-160,共11页Microbiology China

基　　金：国家重点基础研究发展规划项目(973计划)(No.2013CB734004);国家自然科学基金项目(No.31370075;31471725)~~

摘　　要：【目的】通过诱变筛选技术选育阿维菌素高产突变株,对其发酵培养基进行响应面优化,提高阿维菌素产量。【方法】采用常压室温等离子体(ARTP)诱变技术,结合链霉抗性和卡那霉素抗性筛选法及96深孔板高通量筛选法,筛选阿维菌素高产株。在单因素实验的基础上,应用响应面分析法对其发酵培养基进行优化,最后确定最佳培养基配方。【结果】获得一株遗传性状稳定的阿维菌素高产株K-1A6,其阿维菌素产量达到4.22 g/L,比出发菌株9-39提高了23.4%,在最佳培养基中阿维菌素产量达到5.36 g/L,较优化前提高了27.01%。【结论】通过对阿维链霉菌9-39菌株进行ARTP诱变筛选及发酵培养基优化研究能显著提高阿维菌素的产量。[Objective] To improve avermectin yield, mutants of Streptomyces avermitilis 9-39 were screened and the fermentation medium was optimized. [Methods] Atmospheric and room temperature plasma（ARTP） technology was used to treat the spores of S. avermitilis 9-39. The method of streptomycin and kanamycin resistance combined with 96 deep-well plate screening was used for high-throughput screening of high-yielding mutants. Furthermore, based on single factor experiments, the fermentation medium for mutants was optimized by response surface methodology. [Results] A stable avermectin high-producing mutant K-1A6 was obtained and its avermectin production reached 4.22 g/L, 23.4% higher than that of the parent strain 9-39. Under the optimized medium fermentation, the yield of avermectin reached 5.36 g/L, 27.01% higher than that before optimization. [Conclusion] The yield of avermectin can be increased by ARTP mutagenesis, screening and fermentation medium optimization.

关 键 词：阿维菌素 常压室温等离子体诱变技术 抗性筛选 高通量筛选 响应面分析法 

分 类 号：Q93[生物学—微生物学]