利用基因芯片分析活血中药、破血中药对ApoE基因敲除小鼠动脉粥样硬化模型的差异表达基因  

Gene chip analysis of differentially expressed genes in AS model of ApoE mice by blood activating drugs and blood breaking drugs

作　　者：周岚[1] 李杨[1] 汪典[1] 卢青[2] 王敏[2] 陈旭[2] 罗尧岳[1] 谢海波[2] 

机构地区：[1]湖南中医药大学,研究生410208 [2]湖南中医药大学第一附属医院心血管内科,410007

出　　处：《疑难病杂志》2017年第1期18-22,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：国家自然科学基金资助课题(81273752);2013年度高等学校博士学科点专项科研基金资助课题(20134323120007)

摘　　要：目的利用基因芯片技术筛选不同剂量活血中药(川芎、当归)、破血中药(三棱、莪术)对载脂蛋白E(ApoE)基因敲除小鼠动脉粥样硬化(AS)模型胸主动脉组织中差异表达的基因,研究其表达情况并探讨其作用机制。方法以同品系C57BL/6小鼠6只作为空白对照组。将64只5周龄ApoE基因缺陷小鼠按随机数字表法分为模型对照组(n=10)、阿托伐他汀钙组(n=10)、低活血药组(n=11)、低破血药组(n=11)、高活血药组(n=11)、高破血药组(n=11),每日均予普通饲料喂养复制AS模型。造模成功后,空白组和模型组灌服等体积蒸馏水,各组给药剂量分别为:他汀组2.6 mg·kg^(-1)·d^(-1)(相当于阿托伐他汀钙成人日用药量20 mg);低活血组2.6 g·kg^(-1)·d^(-1)(相当于当归、川芎成人日用药量各10 g);低破血组2.6 g·kg^(-1)·d^(-1)(相当于三棱、莪术成人日用药量各10 g);高活血组10.4 g·kg^(-1)·d^(-1)(相当于当归、川芎成人日用药量各40 g);高破血组10.4 g·kg^(-1)·d^(-1)(相当于三棱、莪术成人日用药量各40 g),连续8周,处理动物,用基因芯片技术检测小鼠胸主动脉组织中差异表达的基因。结果空白组与模型组比较,有154个差异表达基因,其中90个基因上调,64个基因下调;模型组与他汀组比较,有96个差异表达基因,其中有17个基因上调,79个基因下调;模型组与低活组比较,有214个差异表达基因,其中95个基因上调,119个基因下调;模型组与低破组比较,有134个差异表达基因,其中87个基因上调,47个基因下调;高活组与低活组比较,有236个差异表达基因,其中169个基因上调,67个基因下调;高破组与低破组比较,有184个差异表达基因,其中88个基因上调,96个基因下调。与AS相关的基因有:与模型组比较,活血药组、破血药组中Cxcr4、Alox12、MoxSap、VcamI的表达下调,Ccr5、Cxcl12、Cxcl16、LCAT的表达上调。结论活血中药(当归、川芎)、破血中药(三棱、莪术)具�Objective Using gene chip technology on load apolipoprotein E （ ApoE） gene in the knockout mice ath-erosclerosis model in thoracic aorta in differentially expressed genes , the expression and discuss the mechanism of different do-ses of drugs for promoting blood circulation （ Ligusticum chuanxiong and Angelica sinensis ） , and blood breaking drugs （ tri, Ezhu） screening.Methods The C57BL/6 mice were used as blank control group （blank group）.64 ApoE gene knock mice with 5 weeks according to the random number table method , were divided into model group （ model group ） , atorvastatin atorv-astatin calcium group （ atorvastatin group ） , low Huoxue treatment group （ low activity group ） , low broken blood medicine group （ low carbon group ） , high blood group （ high activity group ） , high rupture blood drug group （ high rupture group ） , dai-ly were given ordinary feed to duplicate the model of atherosclerosis .After successful modeling , the blank group and the model group were administrated by volume of distilled water , other groups mice were orally administered , 1 times a day, for 8 weeks, in the treatment of animals , with differences in gene chip technology to detect the mouse aortic tissue expression of genes .Re-sults The blank group compared with model group , there were 154 differentially expressed genes , including 90 genes up-reg-ulated and 64 genes were down regulated;the model group compared with atorvastatin group , there were 96 differentially ex-pressed genes , including 17 genes up-regulated and 79 genes were down regulated;the model group compared with the low ac-tivity group, the expression of 214 genes the difference , of which 95 genes were upregulated and 119 genes were down regula-ted;the model group compared with the low carbon group , there were 134 differentially expressed genes , including 87 genes up-regulated and 47 genes down regulated;high activity group compared with the low activity group , there were 236 differenti-ally expressed genes , inc

关 键 词：动脉粥样硬化 活血药 破血药 基因芯片 小鼠 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]