三倍体无籽西瓜子叶节再生体系建立与嫁接育苗技术探究  

Establishment of the cotyledon node regeneration system and vitrografting technology in triploid seedless watermelon

作　　者：闵子扬 阮万辉 张屹 朱菲莹 肖姬玲 李基光 刘建雄 梁志怀 

机构地区：[1]湖南省西瓜甜瓜研究所,长沙410125

出　　处：《中国瓜菜》2017年第1期8-11,共4页China Cucurbits And Vegetables

基　　金：湖南省科技厅重点研发项目(2015NK3047);湖南省科技厅重点研发项目(2016NK2193);国家公益性行业(农业)科研专项(201503110-03)

摘　　要：以三倍体无籽西瓜品种‘超庭三号’子叶节为外植体,对影响子叶节离体再生及嫁接育苗技术的关键因子进行了研究。结果表明,3%的次氯酸钠溶液消毒18 min是三倍体无籽西瓜种胚消毒的最佳方式;2.5 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA是诱导三倍体无籽西瓜子叶节产生不定芽的最佳激素组合,不定芽分化系数最高达6.11;0.2 mg·L^(-1)KT能有效诱导不定芽伸长;将长度为2.5 cm的接穗嫁接到子叶刚展平的南瓜砧木上,成活率高达84.40%。试验成功建立了三倍体无籽西瓜子叶节再生及嫁接育苗技术体系。In this paper, we used seedless watermelon ＇ Chaoting No. 3＇ cotyledonary node as explants for the establish- ment of seedless watermelon cotyledon node regeneration and grafting breeding technology system to investigate the key fac- tors affecting cotyledon node regeneration in vitro and the grafting seedling techniques. The result showed that 3% of sodium hypochlorite solution of 18 min was the best disinfection technique to seedless embryo. 2.5 mg·L-1 6-BA＋0.1 mg·L-1 NAA had the best adventitious bud induction in seedless watermelon, the differentiation coefficient of adventitious bud was as high as 6.11.0.2 mg·L-1 KT could effectively induce adventitious bud to elongate. Scions with 2.5 cm in length which were grated to pumpkin root stocks with flatted cotyledon had the the highest survival rate （84.4%）. In the experiment, we suc- cessfully established the system of cotyledon node regeneration and vitro-grafting technology in triploid seedless watermelon.

关 键 词：无籽西瓜 三倍体 子叶节 再生体系 嫁接 

分 类 号：S651[农业科学—果树学]