OT杂种系百合品种‘Cocossa’小孢子败育细胞学研究  

Cytological Study on Microspore Abortion in OT Hybrid Lily Cultivars 'Cocossa'

作　　者：吴杰[1,2,3] 赵玉萍[1,2,3] 何祥凤[1,2,3] 王文和[1,2,3] 冷平生[1,2,3] 张克中[1,2,3] 

机构地区：[1]北京农学院园林学院,北京102206 [2]北京林果业生态环境功能提升协同创新中心,北京102206 [3]城乡生态环境北京实验室,北京102206

出　　处：《植物研究》2017年第1期15-22,共8页Bulletin of Botanical Research

基　　金：科技创新服务能力建设-协同创新中心-林果业生态环境功能提升协同创新中心(PXM2016_014207_000038);北京市教委;北京市属高等学校创新团队建设与教师职业发展计划(IDHT20150503);北京市教委~~

摘　　要：以OT百合‘Cocossa’为材料,采用醋酸洋红染色烤片法和石蜡切片法,对该品种花粉母细胞减数分裂过程以及绒毡层在花粉母细胞发育过程中的变化进行系统的细胞学观察。研究发现:(1)当百合花蕾长度在2.5~4.5 cm时,花粉母细胞处在减数分裂时期。(2)在花粉母细胞减数分裂的过程中,出现了较多染色体异常现象:中期Ⅰ出现染色体环;后期Ⅰ出现了滞后染色体和染色体桥;在末期Ⅰ和末期Ⅱ细胞出现不均等分裂和微核;处于同一花蕾和同一花药的花粉母细胞减数分裂具有不同步性,能同时观察到2个不同时期的分裂相;形成畸形、无核、多核的异常小孢子。(3)在末期Ⅱ和单核花粉粒时期,绒毡层延迟降解,引起多核花粉粒、畸形花粉粒以及花粉粒自溶现象。结果表明:OT百合‘Cocossa’小孢子败育是花粉母细胞异常减数分裂与绒毡层异常降解共同导致的。We studied meiosis process and tepetum carmine dyeing method combined with grilled slices changes of OT hybrid lily cultivars ＇ Cocossa＇ using aceto- paraffi pollen mother cell was in the period meiosis. There n sections. When the bud grew to 2.5 -4.5 cm, the were some abnormalities appearances of meiosis： chromosome ring appeared in metaphase I , chromosome bridges and lagging chromosomes ppeared in anaphase I , unequal division and micronuclei appeared in telophase I and 11 , it could also be observed 2 split-phase in the same plant, the same flower bud and even the same anther, that showed meiosis had not synchronized. During the telophase I1 and mononuclear pollen period, the tapetum delayed degradation, which led to multinuclear pollen, abnormal pollen and microspore infertility. The meiotic abnormalities and abnormal tapetum degradation could common cause the microspore abortion.

关 键 词：OT百合 减数分裂 绒毡层 小孢子败育 

分 类 号：Q949.718.23[生物学—植物学]