马甲子的三萜类成分研究  

Triterpenoids from Paliurus ramosissimus

作　　者：陈晨[1,2] 罗观堤 付宏征[2] CHEN Chen LUO Guan-di FU Hong-zheng(Department of Pharmacology, General Hospital of Nanj ing Military Area, Nanjing 210002, China State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China Guangdong Sencee Pharmaceutical Co., Ltd., Jieyang 515500, China)

机构地区：[1]南京军区南京总医院药理科,江苏南京210002 [2]北京大学医学部天然药物及仿生药物国家重点实验室,北京100191 [3]广东世信药业有限公司,广东揭阳515500

出　　处：《中草药》2017年第1期36-41,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(81172943)

摘　　要：目的研究鼠李科马甲子属植物马甲子Paliurus ramosissimus中三萜类化学成分。方法通过开放硅胶柱色谱、反相ODS柱色谱、凝胶柱色谱、高效液相色谱等多种方法进行分离、纯化、制备,并通过化合物的理化性质、红外光谱、质谱、核磁共振波谱等方法对化合物进行结构鉴定和解析。结果从马甲子地上部分的95%乙醇水提取物的醋酸乙酯萃取部分中分离得到6个三萜类化合物,分别鉴定为22β,24-二羟基-A(1)-去甲-2,20(29)-羽扇豆二烯-27,28-二羧酸(1)、大枣烯酸(2)、3-O-原儿茶酰美洲茶酸(3)、美洲茶三酸(4)、2-O-咖啡酰麦珠子酸(5)、3-O-咖啡酰麦珠子酸(6)。结论化合物1为新化合物,命名为马甲子酸A,化合物2~6为首次从该植物中分离得到,且首次报道化合物3的完整核磁数据。Objective To study triterpenoids of Paliurus ramosissimus. Methods Six triterpenoids were obtained by modem chromatography technologies, such as repeated chromatography on silica gel, ODS, Sephadex LH-20, and preparative HPLC. Their structures were elucidated on the basis of combination oflR, mass spectrometry, and NMR spectroscopy. Results Six triterpenoids were isolated from the ethyl acetate soluble parts obtained from 95% aq. ethanol extract of the aerial parts of P. ramosissimus. They were identified as 2213,24-dihydroxy-A（ 1 ）-nor-2,20（29）-lupindien-27,28-dicarboxylic acid （1）, zizyberenalic acid （2）, 3-O-protocatechuoylceanothic acid （3）, ceanothetrie acid （4）, 2-O-caffeoylalphitolic acid （5）, and 3-O-caffeoylalphitolic acid （6）. Conclusion Compound 1 paliurus ramosissimus acid A is a new compound. Compounds 2-6 are isolated from P. ramosissimus for the first time. And the complete NMR data of compound 3 has been presented in this paper for the first time.

关 键 词：马甲子 三萜 22β 24-二羟基-A(1)-去甲-2 20(29)-羽扇豆二烯-27 28-二羧酸 马甲子酸A 大枣烯酸 3-O-原儿茶 酰美洲茶酸 美洲茶三酸 2-O-咖啡酰麦珠子酸 

分 类 号：R284.1[医药卫生—中药学]