星点设计-效应面法优选米泔水漂白术炮制工艺  

Optimization of rice-washed water rinsing process technology for Atractylodis Macrocephalae Rhizoma by central composite design-response surface methodology

作　　者：黄小方[1,2] 鄢庆祥 龚鹏飞[1] 于欢[1] 钟凌云[1] 龚千锋[1] HUANG Xiao-fang YAN Qing-xiang GONG Peng-feil YU Huan ZHONG Ling-yun GONG Qian-feng(School of Pharmacology, Jiangxi University ofTCM, Nanchang 330004, China College of Science and Technology, Jiangxi University of TCM, Nanchang 330004, China School of Pharmacology, Guangxi University of TCM, Nanning 530200, China)

机构地区：[1]江西中医药大学药学院,江西南昌330006 [2]江西中医药大学科技学院,江西南昌330006 [3]广西中医药大学药学院,广西南宁530200

出　　处：《中草药》2017年第1期109-113,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助(81460604);江西省卫生厅中医药科研课题(2014Z002)

摘　　要：目的优选樟帮特色米泔水漂白术炮制工艺,为米泔水漂白术炮制规范提供科学依据。方法采用星点设计-效应面法进行米泔水漂白术炮制工艺设计,以米泔水用量、漂洗时间、漂洗温度为考察因素;采用HPLC法测定白术内酯Ⅰ、Ⅱ、Ⅲ等成分量,进行加权综合评分计算OD值(归一化值),采用Design Expert进行2次项回归拟合,优选其最佳工艺并进行验证。结果最佳炮制工艺漂洗条件为米泔水用量为9倍、漂洗时间55 h、漂洗温度26℃,在此条件下,白术内酯Ⅰ、Ⅱ、Ⅲ等成分量综合评分OD值为0.960。结论米泔水漂白术在此条件下稳定可行,能为米泔水漂白术饮片生产与质量标准规范提供参考。Objective To optimize the processing technology ofAtractylodis Macrocephalae Rhizoma prepared by rice-washed water rinsing, and provide a scientific basis for producing specification in processing. Methods Design the processing with central composite design-response surface methodology and take the factors of volume of rice-washed water, rinsing time, and rinsing temperature as independent variables. The contents of atractylenolide Ⅰ、Ⅱ and Ⅲ were determined by HPLC and the comprehensive scores of the three components were regarded as the response index or OD. By analyzing with Design Expert, the best processing parasite for the experiment could be induced. Results The best processing conditions were 9-tirne volume of rice-washed water, 55 h for rinsing, and at the temperature of 26. On the selected condition, the Value of OD was at 0.960. Conclusion The rice-washed water rinsing processing technology for Atractylodis Macrocephalae Rhizoma is stable and feasible under the condition selected, which can be used as reference for its production and quality control.

关 键 词：樟帮 白术 米泔水 漂制 炮制工艺 星点设计-效应面法 白术内酯Ⅰ 白术内酯ⅡⅢ 白术内酯III 

分 类 号：R283.1[医药卫生—中药学]