黄芪和当归配伍对环磷酰胺所致骨髓造血功能抑制小鼠造血功能的影响  

Effects of Astragalus-Angelica compatilibity on bone marrow hematopoiesis suppression induced by Cyclophosphamide in mice

作　　者：黄小平[1] 李菲[1] 陈凌波[1] 张柯胜 邓常清[1] HUANG Xiao-ping LI Fei CHEN Ling-bo ZHANG Ke-sheng DENG Chang-qing(Molecular Pathology Laboratory, Key Laboratory of Hunan Province for Prevention and Treatment of Integrated Traditional Chinese and Western Medicine on Cardio-Cerebral Diseases, Key Laboratory of Hunan Universities for Cell Biology and Molecular Techniques, Hunan University of Chinese Medicine, Changsha 410208, China)

机构地区：[1]湖南中医药大学分子病理实验室,中西医结合心脑疾病防治湖南省重点实验室,细胞生物学与分子技术湖南省高校重点实验室,湖南长沙410208

出　　处：《中草药》2017年第1期121-128,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81473581);湖南省高校科技创新团队“中西医结合防治心脑血管疾病的相关基础研究”;湖南省自然科学创新群体基金“中医药防治心脑血管疾病基础研究”

摘　　要：目的探讨黄芪和当归不同比例配伍对环磷酰胺(CTX)所致骨髓造血功能抑制小鼠造血功能的影响,分析二者配伍的相互作用。方法采用CTX ip复制小鼠骨髓造血功能抑制模型,以重组人粒细胞集落刺激因子(NG-CSF)作为阳性对照药物,药物组分别ig给予当归、黄芪、当归一黄芪不同比例(10:1、5:1、2.5:1、1:1、1:2.5、1:5、1:10)配伍的提取物。测定外周血象,血清造血生长因子粒单系集落刺激因子(GM-CSF)、促红细胞生成素(EPO)、血小板生成素(TPO)的量,计数骨髓有核细胞数(BMNC),测定骨髓造血组织面积及脾指数(SI)。以综合指数法整合所有检测指标,采用多重线性回归分析法分析黄芪、当归2种药物间的相互作用。结果在连续注射CTX后的5~7d,小鼠外周血象显著降低,同时伴有血清GM-CSF、TPO量降低,骨髓BMNC减少,SI升高,骨髓造血组织面积减少。单用黄芪对外周血象、血清造血生长因子(HGF)量、骨髓造血组织面积和BMNC均无显著影响。单用当归可升高外周血中白细胞(WBC)、红细胞(RBC)、血小板(PLT)数,增加骨髓造血组织面积,但能不增加血清HGF的量和BMNC。黄芪-当归(5:1、2.5:1、1:1、1:2.5、1:5、1:10)配伍可升高外周血WBC、RBC、PLT数量,增加HGF的量、BMNC数量和骨髓造血组织面积,降低SI。综合效应分析表明,黄芪-当归1:1、1:2.5、1:5比例配伍时发挥促造血的作用最强。相互作用分析表明,黄芪-当归配伍后,当归发挥促造血作用的重要性大于黄芪,黄芪和当归配伍时其交互作用对药物效应的发挥产生了不可忽视的正面影响,二者配伍对促进造血具有增效作用。结论黄芪-当归配伍具有促进造血的作用,当归的促造血作用大于黄芪,黄芪配伍当归后对促进造血具有协同增效作用,黄芪与当归以1:1、1:2.5、1:5比例配伍时的促造血作用为佳。Objective To investigate the effects of different proportion combinations of Astragalus and Angelica on bone marrow hematopoiesis suppression induced by Cyclophosphamide （CTX） in mice, and to analysis their interactions. Methods The model of bone marrow hematopoietic function suppression in mice was established by ip injection of CTX. Recombinant human granulocyte-macrophage colony-stimulating factor （rhG-CSF） was used as the positive control drug, ICR mice in the drag groups were administered with the extracts of Astragalus, Angelica, and combinations of Astragalus and Angelica with different ratios （10 ： 1, 5 ： 1, 2.5 ： 1, 1 ： 1, 1 ： 2.5, 1 ： 5, and 1：10）. To detect peripheral hemogram, the nucleated cell count in bone marrow （BMNC）, the area of bone marrow hematopoietic tissue, spleen index （SI）, as well as the contents of hematopoietic growth factor （HGF） in serum such as Erythropoietin （EPO）, thrombopoietin （TPO）, and Granulocyte-macrophage colony-stimulating factor （GM-CSF）. To integrate all the test parameters using comprehensive index method, then to analyze the interaction between Astragalus and Angelica through multiple linear regression analysis. Results Following continuous CTX injection for 3 d, peripheral hemogram significantly decreased, accompanied by GM-CSF and TPO contents declined, BMNC reduced, SI elevated, and bone marrow hematopoietic tissue area lessened from day 5 to day 7 after injection. Single Astragalus had no remarkable effects on peripheral hemogram, HGF contents, bone marrow hematopoietic tissue area and BMNC. Except for HGF and BMNC, Angelica alone could increase the numbers ofWBC, RBC, and PLT in the peripheral blood, raise the area of bone marrow hematopoietic tissue. Astragalus mixed Angelica with the ratios of 5 ： 1, 2.5 ： 1, 1 ： 1, 1 ： 2.5, 1： 5, and 1 ： 10 could increase the numbers of WBC, RBC, and PLT along with HGF content, BMNC and bone marrow hematopoietic tissue area, while decreased SI. The comprehensive eff

关 键 词：黄芪 当归 配伍 骨髓抑制 造血 血细胞 造血生长因子 

分 类 号：R285.5[医药卫生—中药学]