地黄属核基因与叶绿体基因DNA条形码比较研究  

Comparison on DNA barcodes of nuclear and chloroplast gene fragments in Rehmannia Libosch.ex Fisch.et Mey.(Rehmanniaceae)

作　　者：李建芳[1] 黄静[1] 程芳婷[1] 杜优[1] 刘占林[1] LI Jian-fang HUANG Jing CHENG Fang-ting DU You LIU Zhan-lin(Key Laboratory of Resource Biology and Biotechnology in Western China （Ministry of Education）, College of Life Sciences, Northwest University, Xi＇an 710069, China)

机构地区：[1]西北大学生命科学学院,西部资源生物与现代生物技术教育部重点实验室,陕西西安710069

出　　处：《中草药》2017年第1期165-171,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(31670219,31370353);陕西省自然科学基础研究计划(2015JM3106);陕西省教育厅项目(11JS093)

摘　　要：目的比较单拷贝核基因、叶绿体基因及ITS等DNA条形码在地黄属的物种分辨率,探讨地黄属种间亲缘关系。方法利用距离法与建树法分析了地黄属植物13个居群的12个单拷贝核基因、6个叶绿体基因以及ITS片段物种鉴定成功率,构建了地黄属系统发育树。结果叶绿体matk和rbc L、单拷贝核基因物种分辨率普遍较低,多基因联合分析有助于物种鉴定成功率的提高。在NJ树中,地黄-茄叶地黄聚为一支,其他物种都是单系群,裂叶地黄位于系统发育树基部。结论 ITS是地黄属物种鉴定的核心条形码,psb A-trnH、trnL-F、trnM-V、trnS-G可以作为备选方案,核基因RR255与R257可用作地黄专用鉴定条形码。四倍体地黄起源方式有待进一步澄清。Objective To compare the usage of single-copy nuclear genes （SCNGs）, chloroplast gene fragments and internal transcribed spacer （ITS） as DNA barcodes in plants ofRehmannia Libosch. ex Fisch. et Mey. and elucidate the interspecific relationships in the genus. Methods Distance and tree-based methods were performed to analyze 12 SCNGs, six cpDNA regions, and ITS fragment in 13 populations of Rehmannia Libosch. ex Fisch. et Mey. Neighbor-joining tree was constructed to investigate the relationships among the species. Results CpDNA regions （matK and rbeL） and SCNGs had lower identification rates than others, especially ITS. Multiple DNA barcodes combination would be helpful to improve the species identification rate. The NJ tree indicated that R. glutinosa and R. solanifolia were clustered together, other species in the genus were monophyletic, and R.piasezla＇i was the basal group. Conclusion ITS should be used as the core barcode in the genus while psbA-trnH, tmL-F, traM-V, and tmS-G would be considered as the candidates, and nuclear genes R255 and R257 might be utilized as unique barcode for the identification ofR. glutinosa. The origin oftetraploid species （R. glutinosa and R. solanifolia） still remains unknown and further works should be done to solve the question.

关 键 词：地黄属 DNA条形码 核基因 叶绿体基因 亲缘关系 

分 类 号：R282.12[医药卫生—中药学]