UPLC结合化学计量法对不同产地黄芪的快速鉴别  

Rapid identification of Astragali Radix from different origins by UPLC combined with chemometrics methods

作　　者：贺敬霞 牟倩倩 张建琪[2,3] 田茜[1] 何晨[1] 尹蓉莉[1] 李化[2] HE Jing-xia MOU Qian-qian ZHANG Jian-qi TIAN Qian HE Chen YIN Rong-li LI Hua(College of pharmacy Chengdu college of TCM, Chengdu 611137, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China Bio and Food Engineering of College, Xihua University, Sichuan, Chengdu 610039, China)

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]中国中医科学院中药研究所,北京100700 [3]西华大学生物食品工程学院,四川成都610039

出　　处：《中草药》2017年第1期179-184,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(81202903;81573534);国家中医药管理局中医药行业科研专项(201407003)

摘　　要：目的建立UPLC-DAD法测定黄芪中4种黄酮类成分的测定方法,结合化学计量学方法快速鉴别黄芪的产地。方法采用UPLC法测定5个省份20批黄芪药材中毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮类成分,运用聚类分析、主成分分析、判别分析方法综合分析不同产地黄芪质量。结果以毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮类成分的量为标准,进行聚类分析与主成分分析,两者结果一致,可将不同黄芪产地区分为3大类。判别分析进一步以Fisher判别函数的形式将内蒙古、四川、吉林产地较准确进行区分,甘肃与陕西的样品出现了误判,初始分组的正确率为85%。结论 UPLC结合化学计量法可快速、准确鉴别黄芪产地,研究结果也可为不同产地黄芪的质量评价提供科学的依据。Objective To determine the contents of four flavonoids inAstragali Radix by UPLC-DAD method, and to rapidly identify their origins using chemometric method. Methods UPLC method was used for determining calycosin-7-O-13-D-glycoside, ononin, calycosin, and formononetin in 20 batches of Astragali Radix from five provinces. Three methods, such as clustering analysis, principal component analysis, and discriminant analysis were compared for comprehensive quality evaluation of Astragali Radix from different places. Results ~rigins^fAstraga^iRadixweresuccessfu~~ydividedint^threecateg^riesbyc^usterana^ysisandprincipa^c~mp^nent analysis, based on the contents of the tested flavonoids. Discriminant analysis could accurately distinguish some origins, such as the Inner Mongolia Autonomous Region and Sichuan and Jilin Provinces, while Shaanxi and Gansu Provinces appeared false classification. The correct rate of the initial group was 85%. Conclusion UPLC combined with chemometric method could be used for rapid and accurate identification of origins of Astragali Radix, which also provided the scientific basis for the quality evaluation of Astragali Radix from different origins.

关 键 词：黄芪 黄酮 UPLC 聚类分析 主成分分析 判别分析 产地 

分 类 号：R286.6[医药卫生—中药学]