人脂肪干细胞与不同浓度纤维蛋白胶的相容性研究  

Compatibility between human adipose derived stem cells and different concentrations of fibrin gel

作　　者：阳水发 易阳艳[1] 黄艳[1] 吴舒[1] 王朝慧[1] 

机构地区：[1]南昌大学第二附属医院医疗美容科,南昌330000

出　　处：《重庆医学》2017年第2期165-168,共4页Chongqing medicine

基　　金：江西省科技支撑计划(2010BSA15100;2010BSB00201);江西省自然科学基金(20151BAB205034)

摘　　要：目的研究人脂肪干细胞(ASCs)与不同浓度纤维蛋白胶支架的体外生物相容性。方法获取人原代ASCs,鉴定后通过CCK-8试验检测纤维蛋白胶对ASCs的毒性。通过光镜直接观察和活细胞/死细胞(DEAD/LIVE)双标染色荧光观察ASCs分别与100.0、50.0、25.0、12.5mg/mL 4组不同浓度纤维蛋白胶支架三维培养后ASCs的生长状况。结果 CCK-8试验显示纤维蛋白胶对ASCs无明显毒性。通过光镜直接观察及DEAD/LIVE双荧光染色后观察显示ASCs与不同浓度纤维蛋白胶复合后细胞呈立体三维生长,ASCs增殖速率和健康程度(DEAD/LIVE值)随纤维蛋白浓度降低而提高,低于25.0mg/mL时细胞增殖速率和健康程度趋于良好。结论 ASCs与纤维蛋白胶支架具有良好的体外生物相容性,纤维蛋白浓度低于25.0mg/mL为与ASCs三维培养的适宜浓度。Objective To research the in vitro compatibility between adipose-derived stem cells（ASCs）and fibrin gel scaffold.Methods The human primary ASCs were obtained and identified.The toxicity of fibrin gel on ASCs was detected by CCK-8test.The light microscopy and DEAD/LIVE double labelled staining fluorescence were used to observe the ASCs growth situation after three dimensional culture of ASCs with 4concentrations of 100.0,50.0,25.0,12.5mg/mL fibrin gel scaffold.Results The CCK-8test showed no significant toxicity of fibrin gel on ASCs.The light microscopy and DEAD/LIVE double labelled staining fluorescence direct observation revealed that ASCs showed a stereo three-dimensional cell growth pattern after cultivation with fibrin gel scaffold,the ASCs proliferation rate and health degree（DEAD/LIVE value）were elevated with the fibrin concentration decrease,in the fibrin concentration less than 25mg/mL,the cellular proliferation rate and health degree tended to be better.Conclusion ASCs and fibrin gel scaffold have favorable in vitro biocompatibility,and the fibrin gel concentration which is less than 25.0mg/mL is appropriate for ASCs three-dimensional culture.

关 键 词：干细胞 脂肪类 纤维蛋白组织黏着剂 组织工程 支架 组织相容性 

分 类 号：R622[医药卫生—整形外科]