阿司匹林对不稳定型心绞痛患者血浆中5种miRNA水平的影响  

The effect of aspirin on the expression of five miRNA in plasma of patients with unstable angina pectoris

作　　者：李云[1,2] 吕云波[2] 宋银宏[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学人民医院心内科,湖北宜昌443002

出　　处：《重庆医学》2017年第2期178-181,184,共5页Chongqing medicine

基　　金：湖北省教育厅自然科学研究项目(Q20121306)

摘　　要：目的探讨口服阿司匹林对不稳定心绞痛(UAP)患者血浆miR-21、miR-24、miR-126、miR-146、miR-223水平的影响。方法以未服用过阿司匹林的15例冠状动脉病变阳性的UAP患者及10例冠状动脉病变阴性的UAP患者作为研究对象,予以阿司匹林肠溶片0.1g,每日1次口服,实时定量反转录聚合酶链反应(RT-qPCR)检测miRNA浓度,比较服药前及服药1周后外周血浆中这5种miRNA的差异。同时选取50例UAP患者(包括冠状动脉病变阴性及阳性者),根据其近期是否服用阿司匹林情况分为阿司匹林组及对照组,比较不同组间血浆中5种miRNA水平。结果仅冠状动脉病变阳性的UAP患者服用阿司匹林后血浆中的miR-223水平升高有统计学意义(P<0.05),而其他几种miRNA及冠状动脉病变阴性者血浆中5种miRNA水平变化均无统计学意义(P>0.05)。结论阿司匹林可升高冠状动脉病变阳性的UAP患者血浆中miR-223的水平但不影响冠状动脉病变阴性者血浆miR-223水平。Objective To detect the effect of aspirin on the expression of miR-21,miR-24,miR-126,miR-146 and miR-223 in plasma of patients with unstable angina pectoris（UAP）.Methods Fifteen UAP patients with coronary artery disease and ten UAP patients without coronary artery disease were selected as subjects.All subjects had no medication of aspirin before.Aspirin entericcoated tablets was taken to all subjects at 0.1g once a day orally for one week.The peripheral blood（PB）samples of the patients were collected before and after taking the drug for one week.The expressions of the 5miRNAs in plasma were detected by quantitative reverse transcription PCR（RT-qPCR）.Meanwhile,fifty UAP patients with or without coronary artery disease were divided into the aspirin group and the control group according to the medication of aspirin.The expressions of the 5miRNAs in plasma between different groups were compared.Results The expression of miR-223 in plasma of UAP patients with coronary artery disease was increased significantly after the medication of aspirin（P〈0.05）.However,the expression of the other 4miRNAs in plasma of these UAP patients with coronary artery disease and the 5miRNAs in plasma of the UAP patients without coronary artery disease did not change significantly before and after the medication of aspirin（P〉0.05）.Conclusion The medication of aspirin is able to increase the expression of miR-223 in serum of UAP patients with coronary artery disease but not of UAP patients without coronary artery disease.

关 键 词：阿司匹林 冠状动脉疾病 心绞痛 不稳定型 微RNAS 

分 类 号：R541.4[医药卫生—心血管疾病]