猪抑胃肽/葡萄糖依赖性促胰岛素释放肽(GIP)基因的克隆与组织表达分析  

Cloning and tissue expression of gastric inhibitory polypeptide/glucosedependent insulinotropic polypeptide(GIP) in pigs

作　　者：程绍臣[1] 张剑南[1] 钟宇[1] 李娟[1] 王亚军[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2017年第1期161-166,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家转基因生物新品种培育重大专项(2009zx08009-145B)

摘　　要：本研究采用RT-PCR方法,克隆长白猪(Landrace)GIP基因全长cDNA序列,并对其在家猪组织中的表达情况进行分析.结果显示:长白猪GIP(pGIP)cDNA全长435bp,编码144个氨基酸GIP的前体蛋白;该前体蛋白含有信号肽序列,经蛋白酶水解后产生GIP成熟肽.与人、小鼠GIP表达图谱类似,GIP mRNA也在长白猪小肠及肾脏中有较高水平表达.The gastric inhibitory polypeptide/glucose-dependent insulinotropic polypeptide （GIP） shows the inhibitory effect on gastric acid secretion and incretin action. The aim of this study is to analyze the cDNA sequence of the pig GIP （pGIP） gene, and further examine the tissue expression of pGIP based on RT-PCR method. Result showed that pGIP cDNA is 532 bp in length and encodes a 144-amino acid GIP precursor which contains a signal peptide. RT-PCR assay showed that pGIP mRNA is highly expressed in kidney and gastrointestinal tract. Characterization of the pGIP gene will help to elucidate its physiological role.

关 键 词：猪 GIP 分子克隆 结构分析 组织表迭 

分 类 号：Q78[生物学—分子生物学] S828[农业科学—畜牧学]