成都动物园野生动物源贾第虫的多位点基因分型鉴定  

Multilocus genotype(MLG) analysis of Giardiafrom captive wildlife in Chengdu zoo

作　　者：李威[1] 屈羽[2] 钟志军[1] 杨平 李云娇[1] 王吴优 刘学涵[1] 谢娜[1] 邓家波[2] 彭广能[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,温江611130 [2]成都动物园成都野生动物研究所,四川成都610081 [3]四川省雅安市名山区农业局,四川名山625100

出　　处：《四川大学学报（自然科学版）》2017年第1期185-190,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金项目(31272620);成都动物园项目(2014-04)

摘　　要：为了解四川省成都市动物园野生动物贾第虫的流行及基因型,本研究采集了146份不同野生动物的新鲜粪便并提取基因组DNA.通过巢式PCR扩增β-giardin、tpi和gdh基因,扩增产物测序后进行种系发育分析.结果表明,CDZOO1黇鹿源和CDZOO3龟源贾第虫通过多位点基因分型(MLG)鉴定为AI-1亚型;CDZOO2鹿源贾第虫为E型(β-giardin基因位点);CDZOO4黇鹿源贾第虫为A型(β-giardin和tpi基因位点);CDZOO5浣熊源和CDZOO6细尾獴源贾第虫在β-giardin位点为D型而在tpi位点为A型.In order to investigate the infection and genotypes of Giardia from different wildlife in Chengdu zoo, a total of 146 fresh fecal samples were collected and their genome DNA extracted. The β-giardin, tpi and gdh genes were amplified by nested-PCR and the product were sequenced followed by phylogenetic analysis. As a result, two persian fallows （CDZOO1 and CDZOO4）, a deer （CDZOO2）, a tortoise （CDZOO3）, a raccoon （CDZOO5）, a meerkat （CDZOO6） were infected with Giardia. Multilocus genotypes （MLGs） identified assemblages AI-1 in CDZOO1 and CDZOO3 ; CDZ002 was infected with as- semblage A in both β-giardin and tpi loci; CDZOO4 was confirmed as assemblage A at the tpi locus; CDZOO5 and CDZOO6 were identified as assemblage D at the β-giardin locus while assemblage A based on the tpi locus.

关 键 词：贾第虫 β-giardin基因 tpi基因 gdh基因 MLG 种系发育 人畜共患 

分 类 号：Q78[生物学—分子生物学]